{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T06:45:15Z","timestamp":1782715515822,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The mouse estrous cycle drives cyclical remodelling of the endometrium, essential for uterine function and embryo implantation. However, the molecular mechanisms orchestrating these dynamic changes remain incompletely defined. In this study, we preliminarily staged C57BL\/6 mice using vaginal smear cytology and confirmed phase\u2010dependent morphological changes, including cyclical endometrial thickening and glandular remodelling, via histological assessment. To elucidate the underlying molecular landscape, we performed comprehensive RNA sequencing of the mouse endometrium across four distinct phases: proestrus, estrus, metestrus and diestrus. To ensure analytical rigour and identify core regulatory factors, we integrated three synergistic computational approaches: WGCNA to identify phase\u2010specific co\u2010expression modules, MFUZZ to characterize dynamic temporal expression trajectories, and DESeq2 to pinpoint significantly differentially expressed genes (DEGs). Functional enrichment analysis revealed that these phase\u2010specific signatures are predominantly involved in the cell cycle, extracellular matrix organization, oxidative phosphorylation and DNA replication. Notably, we identified several high\u2010connectivity hub genes and pathways, including Natural Killer (NK) cell\u2010mediated cytotoxicity (\n                    <jats:italic>Cd244a<\/jats:italic>\n                    ,\n                    <jats:italic>Klra4<\/jats:italic>\n                    ,\n                    <jats:italic>Klrd1<\/jats:italic>\n                    ), Oestrogen signalling (\n                    <jats:italic>Ccnd1<\/jats:italic>\n                    ,\n                    <jats:italic>Akt1<\/jats:italic>\n                    ,\n                    <jats:italic>Prkaca<\/jats:italic>\n                    ) and Wnt signalling components (\n                    <jats:italic>Cdh1<\/jats:italic>\n                    ,\n                    <jats:italic>Skp1a<\/jats:italic>\n                    ,\n                    <jats:italic>Pax2<\/jats:italic>\n                    ). Transcription factors such as\n                    <jats:italic>Sox4<\/jats:italic>\n                    and\n                    <jats:italic>Lef1<\/jats:italic>\n                    , along with the cell cycle regulator\n                    <jats:italic>Cks1b<\/jats:italic>\n                    , exhibited phase\u2010consistent expression patterns aligned with endometrial proliferation and preparation for implantation. Our findings delineate a complex hub\u2010gene regulatory network coordinating cyclic endometrial regeneration. This study provides a comprehensive transcriptomic atlas of the murine endometrium, offering novel insights into uterine biology with significant implications for understanding reproductive health and uterine diseases.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.71265","type":"journal-article","created":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T04:13:04Z","timestamp":1782533584000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dynamic Transcriptomic Profiling of Mouse Endometrium Across the Estrous Cycle Reveals Phase\u2010Specific Regulatory Networks Underlying Cyclic Remodelling"],"prefix":"10.1111","volume":"30","author":[{"given":"Xinyan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Gynaecology and Obstetrics The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine)  Hangzhou China"},{"name":"The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keyi","family":"Xu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine)  Hangzhou China"},{"name":"Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binting","family":"Li","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine)  Hangzhou China"},{"name":"Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotong","family":"Xu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine)  Hangzhou China"},{"name":"Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3967-493X","authenticated-orcid":false,"given":"Gang","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Cell\u2010Based Drug and Applied Technology Development in Zhejiang Province Institute for Cell\u2010Based Drug Development of Zhejiang Province, S\u2010Evans Biosciences  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Cell\u2010Based Drug and Applied Technology Development in Zhejiang Province Institute for Cell\u2010Based Drug Development of Zhejiang Province, S\u2010Evans Biosciences  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0810-3392","authenticated-orcid":false,"given":"Ruolang","family":"Pan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Cell\u2010Based Drug and Applied Technology Development in Zhejiang Province Institute for Cell\u2010Based Drug Development of Zhejiang Province, S\u2010Evans Biosciences  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine)  Hangzhou China"},{"name":"The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom15030450"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2416089122"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00035.2023"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm14175965"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJWH.S481509"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202500961RR"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12663"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964\u2010022\u201001018\u20108"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/17501911.2025.2491298"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.107993"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adu3699"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/lifemedi\/lnae041"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1070"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.01.021"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25179429"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13151236"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26020857"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032\u2010024\u201001750\u20101"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020\u2010024\u201001054\u20109"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958\u2010023\u201001135\u20100"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2422694122"},{"issue":"6","key":"e_1_2_10_24_1","first-page":"899","article-title":"Sequential Changes in the Expression of Wnt\u2010 and Notch\u2010Related Genes in the Vagina and Uterus of Ovariectomized Mice After Estrogen Exposure","volume":"26","author":"Nakamura T.","year":"2012","journal-title":"In Vivo"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040312"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010025\u201007880\u20109"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgwh.2022.856316"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13353\u2010025\u201000939\u20107"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2024.103205"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25126473"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.202317601"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells14010058"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420\u2010024\u201002228\u20104"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/359076a0"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.24.11408"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.1223"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.51139"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqae049"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqad169"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.71265","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.71265","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.71265","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T05:55:55Z","timestamp":1782712555000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.71265"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":38,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/jcmm.71265"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.71265","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2026-02-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71265"}}