{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T07:26:23Z","timestamp":1786778783249,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["GZC20233579"],"award-info":[{"award-number":["GZC20233579"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Radiation\u2010induced intestinal injury (RIII) is among the most common complications of radiotherapy in patients with abdominaltumours. At present, there are no effective methods for reducing the occurrence or severity of RIII. Berberine (BBR) is a quaternary ammonium alkaloid extracted from\n                    <jats:italic>Coptis chinensis<\/jats:italic>\n                    that has antioxidant, anti\u2010inflammatory, and protective effects on the intestine. A RIII model was established using 10\u2009Gy of X\u2010ray total abdominal irradiation (TAI). The effects of BBR on mice exposed to 10\u2009Gy of X\u2010ray TAI were determined by analysing pathological sections of the mouse intestine. TUNEL staining was used to detect apoptosis of intestinal epithelial cells in mice. Immunohistochemistry was applied to detect the expression of proliferation indicators PCNA and Ki\u201067, and immunofluorescence staining was used to quantify BrdU\u2010positive proliferative cells. BBR stimulates crypt formation ex\u00a0vivo after irradiation and upregulates the expression of FXR. The expression of goblet cell and intestinal stem cell markers was quantified by qRT\u2010PCR. The expression of farnesoid X receptor (FXR) in RIII was detected via qRT\u2010PCR and Western blot. Additionally, a Western blot was performed to detect the protein levels of NF\u2010\u03baB, p\u2010p38, total p38, and \u03b2\u2010catenin, and qRT\u2010PCR was used to measure the mRNA expression levels of pro\u2010inflammatory factors TNF\u2010\u03b1, IL\u20101\u03b2, and IL\u20106. BBR alleviated RIII, mainly manifested as body weight loss, longer colons, greater numbers of villi, and greater numbers of crypts. BBR also maintained the regeneration ability and promoted the proliferation of crypt cells, reduced the apoptosis rate, and alleviated intestinal injury. Importantly, BBR rescued radiation\u2010induced dysregulation of these key signalling proteins and pro\u2010inflammatory factors. BBR failed to promote the repair of RIII when FXR was inhibited. BBR treatment increased the expression of FXR in crypts and was at least protective against radiation\u2010induced intestinal damage in mice through the modulation of FXR. BBR may be a potential drug for the treatment of radiation\u2010induced intestinal damage.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.71281","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T04:16:38Z","timestamp":1786335398000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Role and Mechanism of Berberine as a Candidate Drug for the Treatment of Radiation\u2010Induced Intestinal Injury"],"prefix":"10.1111","volume":"30","author":[{"given":"Xihao","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Environmental, Health Hazard Assessment and Protection of Shaanxi Province Key Laboratory of Free Radical Biology and Medicine of Shaanxi Province  Xi'an 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