{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T04:59:19Z","timestamp":1785301159176,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82374486"],"award-info":[{"award-number":["82374486"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004863","name":"Zhejiang Chinese Medical University","doi-asserted-by":"publisher","award":["Y202351312"],"award-info":[{"award-number":["Y202351312"]}],"id":[{"id":"10.13039\/501100004863","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Osteoporosis (OP) is a prevalent skeletal disorder characterized by progressive bone mass loss and deteriorated microarchitecture, in which oxidative stress\u2010induced mesenchymal stem cell (MSC) dysfunction serves as a core pathogenic mechanism. Yougui Pills (YGPs), a classical traditional Chinese medicine formula, are widely applied in clinical OP management, yet the cellular and molecular mechanisms underlying their anti\u2010osteoporotic effects remain incompletely defined. This study aimed to elucidate the bone\u2010protective role of YGPs in OP and explore the underlying mechanism, focusing on oxidative stress modulation in MSCs and the Nrf2\/HO\u20101 signalling pathway. The bioactive components of YGPs and YGP\u2010containing serum were characterized via UPLC. In vivo, an ovariectomy (OVX) mouse model was established to evaluate YGPs' effects on bone mass, trabecular microstructure and osteogenesis using micro\u2010CT, histological and immunohistochemical assays; public GEO datasets were re\u2010analysed to profile transcriptomic alterations and oxidative stress signatures in OP\u2010derived MSCs. In vitro, H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    was used to induce ROS accumulation and oxidative injury in MSCs, with assessments of cell proliferation, apoptosis, ROS levels, migration and osteogenic differentiation. Network pharmacology and siRNA\u2010mediated gene silencing were conducted for target prediction and mechanistic validation. In vivo results showed that YGP treatment significantly ameliorated OVX\u2010induced osteopenia, increased bone mineral density, improved trabecular microstructure, and upregulated osteogenic markers (ALP, OCN, Runx2, COL1A1). Transcriptomic re\u2010analysis revealed upregulated oxidative stress markers in OP MSCs, consistent with In vitro findings that YGPs attenuated H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010triggered ROS overproduction, suppressed apoptosis and ectopic lipid deposition, and enhanced MSC proliferation, migration and osteogenic differentiation. Mechanistically, YGPs activated the Nrf2\/HO\u20101 signalling axis, while Nrf2 knockdown abrogated YGPs' cytoprotective and pro\u2010osteogenic effects. In conclusion, YGPs mitigate oxidative stress\u2010induced MSC dysfunction and promote osteogenesis via the Nrf2\/HO\u20101 pathway, supporting YGPs as a promising therapeutic candidate for OP.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.71295","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T05:03:30Z","timestamp":1784783010000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Yougui Pills Alleviate Osteoporosis by Inhibiting Mesenchymal Stem Cell\n                    <scp>ROS<\/scp>\n                    Accumulation via the Nrf2\/\n                    <scp>HO<\/scp>\n                    \u20101 Pathway"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5808-9974","authenticated-orcid":false,"given":"Jiangyuan","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Orthopaedics and Traumatology of Zhejiang Province  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