{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T07:26:22Z","timestamp":1786778782955,"version":"build-2736575974"},"reference-count":72,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We investigated the potential of miR\u2010640 to downregulate CDK4\/6 inhibitor resistance\u2010associated genes and evaluated its apoptotic, anti\u2010metastatic and anti\u2010proliferative effects, both alone and in combination with abemaciclib in breast cancer cells. miR\u2010640 was identified via GSE126125 dataset analysis. Targets were predicted using miRDB, TargetScan and TargetMiner, filtering for resistance\u2010associated genes. MTT assays determined IC50 values for abemaciclib and combinations. Functional effects were evaluated in MCF7 and MDA\u2010MB\u2010231 cells through Annexin V, cell cycle and wound healing assays. Target gene expression was quantified by RT\u2010qPCR. Successful transfection significantly upregulated miR\u2010640 (~50\u2010fold). The miR\u2010640\/abemaciclib combination strongly inhibited migration, induced apoptosis and triggered cell line\u2010specific phase arrests (S and G2\/M in MCF7; G0\/G1 in MDA\u2010MB\u2010231). Gene expression analysis showed significant downregulation of a comprehensive resistance network (including\n                    <jats:italic>CDK4\/6<\/jats:italic>\n                    ,\n                    <jats:italic>CDKN2B<\/jats:italic>\n                    ,\n                    <jats:italic>WNT7B<\/jats:italic>\n                    ,\n                    <jats:italic>MAP3K1<\/jats:italic>\n                    and\n                    <jats:italic>AURKA<\/jats:italic>\n                    ) in MCF7 cells. In MDA\u2010MB\u2010231 cells,\n                    <jats:italic>PDK1<\/jats:italic>\n                    ,\n                    <jats:italic>CDK6<\/jats:italic>\n                    ,\n                    <jats:italic>CDKN2B<\/jats:italic>\n                    ,\n                    <jats:italic>SMAD2<\/jats:italic>\n                    ,\n                    <jats:italic>ZFP91<\/jats:italic>\n                    ,\n                    <jats:italic>MAP3K1<\/jats:italic>\n                    and\n                    <jats:italic>AURKA<\/jats:italic>\n                    were significantly suppressed. This study identifies miR\u2010640 as a potent tumour suppressor that resensitises breast cancer cells to CDK4\/6 inhibition. By post\u2010transcriptionally downregulating key resistance genes, miR\u2010640\u2014alone or additively with abemaciclib\u2014suppresses proliferation and migration while inducing apoptosis, emerging as a promising combinatorial therapeutic strategy.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.71298","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T12:46:34Z","timestamp":1786365994000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>miR<\/scp>\n                    \u2010640 Enhances Abemaciclib Sensitivity by Suppressing\n                    <scp>CDK<\/scp>\n                    Inhibitor Resistance\u2010Associated Genes in Breast Cancer Cells"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0847-7738","authenticated-orcid":false,"given":"Ismail Mert","family":"Alkac","sequence":"first","affiliation":[{"name":"Department of Medical Biology Ege University Medical School  \u0130zmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8251-4520","authenticated-orcid":false,"given":"Cigir","family":"Biray Avci","sequence":"additional","affiliation":[{"name":"Department of Medical Biology Ege University Medical School  \u0130zmir 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