{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T07:07:21Z","timestamp":1789456041952,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009110","name":"Natural Science Foundation of Xinjiang Uygur Autonomous Region","doi-asserted-by":"publisher","award":["2023D01C98"],"award-info":[{"award-number":["2023D01C98"]}],"id":[{"id":"10.13039\/100009110","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Glioblastoma progression is driven by stemness acquisition and cellular plasticity, yet the temporal and spatial organization of these processes remains incompletely defined. In this study, single\u2010cell RNA sequencing, pseudotime reconstruction, spatial transcriptomics, and in\u00a0vitro and in\u00a0vivo functional assays were integrated to delineate the role of SOX9 during glioblastoma stemness acquisition. SOX9 expression peaked during the early astrocyte\u2010to\u2010malignant transition and declined after malignant states became stabilized. Along pseudotime, SOX9 was inversely associated with the upstream Hippo kinase module and showed phase\u2010dependent coupling with YAP\/TAZ\u2010associated transcriptional programmes. Spatial analyses further revealed marked regional heterogeneity, with the strongest SOX9\u2010malignant coupling observed in the perivascular niche. Functionally, SOX9 gain and loss produced reciprocal changes in glioblastoma cell proliferation, migration, invasion, apoptosis, and xenograft growth, while pharmacological modulation of Hippo signalling partially rescued the effects induced by SOX9 loss. Consistent pathway\u2010level changes were also observed in YAP\/TAZ expression and p\u2010YAP\/YAP and p\u2010MOB1\/MOB1 ratios, and xenograft histology showed SOX9\u2010associated morphological and CD68\u2010positive cell changes. These findings identify SOX9 as a temporally restricted regulator of glioblastoma stemness acquisition and support an early priming\u2010late decoupling model of Hippo\u2010YAP\/TAZ rewiring, providing a rationale for stage\u2010specific and niche\u2010aware therapeutic targeting in glioblastoma.<\/jats:p>","DOI":"10.1111\/jcmm.71341","type":"journal-article","created":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T14:25:41Z","timestamp":1788099941000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Early\n                    <scp>SOX9<\/scp>\n                    Activation Primes Hippo\u2013\n                    <scp>YAP<\/scp>\n                    \/\n                    <scp>TAZ<\/scp>\n                    Rewiring During Glioblastoma Stemness Acquisition"],"prefix":"10.1111","volume":"30","author":[{"given":"Maimaitili","family":"Mijiti","sequence":"first","affiliation":[{"name":"Neurosurgery Centre, 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