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We performed single\u2010cell RNA sequencing on peripheral blood mononuclear cells from eight TB patients, stratified by 2\u2010month SCC status. Non\u2010responders showed a relative enrichment of non\u2010classical monocytes and higher TB progression risk scores. CellChat analysis inferred altered IL16 and TRAIL communication involving these cells. CD4+ Tregs in non\u2010responders showed higher LGALS9 expression and inferred GALECTIN signalling towards cytotoxic lymphocyte subsets; cell\u2010level LGALS9 co\u2010expression correlated with HAVCR2 and TIGIT in non\u2010responders. Mature NK subclusters showed distinct enrichment profiles. These results describe an observational, transcriptomic and computationally inferred immune network associated with early treatment non\u2010response. They generate candidate biomarkers and hypotheses for prospective protein\u2010level and functional validation.<\/jats:p>","DOI":"10.1111\/jcmm.71345","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:24:15Z","timestamp":1788236655000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Single\u2010Cell Immune Profiling Suggests Non\u2010Classical Monocyte Is Associated Immunodepression in Tuberculosis Treatment Non\u2010Responders"],"prefix":"10.1111","volume":"30","author":[{"given":"Yiqun","family":"Xiong","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases Ningbo Hospital of Integrated Traditional Chinese and Western Medicine  Ningbo Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yahong","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Ningbo Hospital of Integrated Traditional Chinese and Western Medicine  Ningbo Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Ningbo Hospital of Integrated Traditional Chinese and Western Medicine  Ningbo Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Tuberculosis Prevention and Control, Ningbo Municipal Center for Disease Control and Prevention  Ningbo Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Shen","sequence":"additional","affiliation":[{"name":"Cosmos Wisdom Biotech Co. 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