{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T07:07:23Z","timestamp":1789456043036,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82274521"],"award-info":[{"award-number":["82274521"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by skin barrier dysfunction and immune balance disorders. Currently, drugs for repairing the skin barrier in AD are limited. The gu\u2010ben\u2010hua\u2010shi formula (AESS) has been shown to regulate Th1\/Th2\/Th17\/Treg imbalance with good clinical effect. However, its effects on regulating autophagy, repairing the skin barrier, and interacting with Yes\u2010associated protein (YAP) remain unknown. To elucidate the therapeutic effects of AESS on AD and to investigate its mechanism of restoring skin barrier function through the Hippo\u2010YAP pathway. We conducted a single\u2010arm observational study to evaluate the clinical efficacy of the AESS in patients with AD. Network pharmacology was used to predict the potential therapeutic targets and mechanisms. AD mouse models were established by house dust mite stimulation in Nishiki\u2010nezumi Cinnamon\/Nagoya mice, and the effects on autophagy and the skin barrier were measured using western blotting, immunofluorescence, mRFP\u2010GFP\u2010LC3 tandem fluorescence assay, and transmission electron microscopy. The effects of AESS serum on an AD\u2010like inflammatory cell model were detected in HaCaT cells stimulated with interferon\u2010\u03b3 (IFN\u2010\u03b3)\/tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1). Activity of the Hippo\u2010YAP signalling pathway was also investigated after YAP knockdown. AESS improved skin rash and barrier function, reduced eosinophil counts, and increased Th1\/Th2 and Treg\/Th17 ratios in patients with AD. AESS increased autophagy, improved skin barrier function, and inhibited Hippo\u2010YAP signalling pathway activity. YAP knockdown reduces these therapeutic effects. Besides regulating immune imbalance, AESS increases keratinocyte autophagy levels, thereby restoring skin barrier function in AD through the Hippo\u2010YAP signalling pathway.<\/jats:p>\n                  <jats:p>\n                    <jats:bold>Trial Registration:<\/jats:bold>\n                    The Chinese clinical trial registration website: ITMCTR202500042\n                  <\/jats:p>","DOI":"10.1111\/jcmm.71361","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T07:21:02Z","timestamp":1789197662000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mechanism of Gu\u2010Ben\u2010Hua\u2010Shi (\n                    <scp>AESS<\/scp>\n                    ) Formula Regulating Autophagy Through Hippo\u2010\n                    <scp>YAP<\/scp>\n                    Pathway in Restoring the Skin Barrier in Atopic Dermatitis"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9472-2660","authenticated-orcid":false,"given":"Xin","family":"Ma","sequence":"first","affiliation":[{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome\/State Key Laboratory of Dampness Syndrome of Chinese Medicine\/Department of Dermatology The Second Clinical College of Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1610-7759","authenticated-orcid":false,"given":"Yiting","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome\/State Key Laboratory of Dampness Syndrome of Chinese Medicine\/Department of Dermatology The Second Clinical College of Guangzhou University of Chinese Medicine  Guangzhou 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