{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:46:08Z","timestamp":1791996368374,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81760096"],"award-info":[{"award-number":["81760096"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Remote ischaemic preconditioning (RIPC) protects against renal ischaemia\u2013reperfusion injury (IRI), yet its precise mechanism remains incompletely understood, particularly regarding the role of mitochondrial quality control. This study investigated whether SIRT3\u2010dependent mitophagy mediates the protective effects of RIPC against acute kidney injury (AKI) induced by IRI and further examined the integrity of mitophagic flux and involvement of the classic Parkin\u2010mediated mitophagic pathway. We used a murine bilateral renal ischaemia\u2013reperfusion injury (IRI) model, including RIPC pretreatment, 3\u2010methyladenine (3\u2010MA) intervention and SIRT3 knockout intervention, and a HK\u20102 cell hypoxia\/reoxygenation (H\/R) model, including SIRT3 siRNA knockdown, SIRT3 overexpression and chloroquine (CQ) intervention. Renal function, histopathological changes, apoptosis, mitochondrial membrane potential and mitophagy were assessed using biochemical analysis, histological and TUNEL staining, JC\u20101 staining, transmission electron microscopy, mitochondrial protein analysis and immunofluorescence colocalization of Parkin and LC3B. RIPC improved renal function, alleviated tubular injury and apoptosis, and enhanced mitophagy in mice, which was attenuated by SIRT3 knockout or 3\u2010MA. In HK\u20102 cells, RIPC plasma protected against H\/R injury and activated mitophagic flux, as verified by CQ treatment, while SIRT3 knockdown abolished these effects and SIRT3 overexpression maintained protective mitophagy levels. RIPC pretreatment restored the IRI\u2010induced reduction in Parkin and LC3B colocalization in renal tubular epithelial mitochondria, and this effect was attenuated by 3\u2010MA. RIPC alone had no detectable cytotoxicity or effect on basal mitophagy. In conclusion, RIPC mitigates renal IRI by activating SIRT3\u2010dependent mitophagy, with involvement of the Parkin\u2010mediated mitophagic pathway. Our in\u00a0vivo and in\u00a0vitro data support the central role of SIRT3 in RIPC\u2010induced renoprotection and provide evidence for an intact mitophagic flux mechanism.<\/jats:p>","DOI":"10.1111\/jcmm.71372","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T03:23:38Z","timestamp":1791775418000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>RIPC<\/scp>\n                    Protects Against Renal Ischaemia\u2013Reperfusion Injury by Activating\n                    <scp>SIRT<\/scp>\n                    3\/Parkin\u2010Mediated Mitophagy"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6658-6867","authenticated-orcid":false,"given":"Qian","family":"Cui","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine The First Affiliated Hospital of Baotou Medical College  Baotou 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Medical College  Baotou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6428-6069","authenticated-orcid":false,"given":"Chenghui","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Beijing Luhe Hospital, Capital Medical University  Beijing China"},{"name":"Department of Intensive Care Medicine Beijing Luhe Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanjun","family":"Pei","sequence":"additional","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of Baotou Medical College  Baotou 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