{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T05:44:28Z","timestamp":1792215868201,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Peripheral arterial disease (PAD) impairs limb perfusion, leading to myopathy characterised by altered mitochondrial content and respiration. Because ischemic muscle appears to adapt differently across PAD stages, we examined stage\u2010specific skeletal\u2010muscle mitochondrial function before and after successful revascularisation. Muscle biopsies of gastrocnemius and vastus lateralis muscle were obtained from 32 patients\u201421 with intermittent claudication (IC) and 11 with chronic limb\u2010threatening ischemia (CLTI)\u2014before and 6\u2009weeks after successful revascularisation. Mitochondrial respiration was assessed by high resolution respirometry (HRR). HRR results were normalised to citrate synthase activity (CSA) as a marker of mitochondrial content. Inter\u2010individual variation was expressed as the gastrocnemius\u2010to\u2010vastus respiration ratio (\u2018GV ratio\u2019). In the IC group, CSA\u2010normalised mitochondrial respiration of gastrocnemius muscle was significantly reduced after revascularisation, paralleled by a significant decline in the GV ratio. In contrast, CLTI patients showed no significant pre\u2010 to postoperative change in CSA\u2010normalised respiration or GV ratio, but mitochondrial content significantly improved by revascularisation. Mitochondrial respiration 6\u2009weeks after successful revascularisation adapts only modestly in milder PAD stages such as IC, while more advanced stages (CLTI) display no functional adaptations. These findings suggest that mitochondrial adaptive capacity is stage\u2010dependent and may require targeted therapeutic strategies beyond revascularisation alone, especially in CLTI.<\/jats:p>","DOI":"10.1111\/jcmm.71383","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T05:20:58Z","timestamp":1792214458000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chronic Limb\u2010Threatening Ischemia Shows No Adaptability of Mitochondrial Function After Revascularisation"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0048-0943","authenticated-orcid":false,"given":"Fiona","family":"Speichinger","sequence":"first","affiliation":[{"name":"Department of General and Visceral Surgery Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin 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