{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:45:51Z","timestamp":1791996351634,"version":"4.4.0"},"reference-count":68,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ya\u2010Jie\u2010Sha\u2010Ba decoction (YJSB), a traditional Dai medicinal formulation, has been widely used by the Dai ethnic group and has shown efficacy in alleviating alcohol\u2010associated liver disease (ALD). However, the molecular mechanism of YJSB in the management of ALD remains unclear. Building on evidence that YJSB ameliorates ALD, we combined transcriptomics and untargeted metabolomics to elucidate the molecular mechanisms responsible for its anti\u2010ALD effects. We established an ALD mouse model and treated animals with YJSB to evaluate its therapeutic effects. We then performed integrated transcriptomic and untargeted metabolomic analyses to identify pathways and molecules associated with YJSB treatment. Using UPLC\u2010Q\u2010TOF\u2010MS, we identified YJSB prototype components present in plasma. We screened the major plasma\u2010detected constituent for hepatoprotective activity in an in\u00a0vitro model of alcohol\u2010induced hepatocyte injury. Finally, we validated the molecular target of this active component using molecular docking, cellular thermal shift assay (CETSA), drug affinity responsive target stability (DARTS), and pharmacological inhibition assays. The results showed that YJSB markedly reduced hepatic injury and lipid accumulation in ALD mice and simultaneously decreased oxidative stress and inflammation. Integrated multi\u2010omics analysis identified arachidonic acid metabolism as a potential pathway mediating YJSB's protective effects. In particular, YJSB robustly increased the expression of CYP4A14, a key enzyme in this pathway. We identified methyl palmitate as the major active component of YJSB that mitigated alcohol\u2010induced hepatocyte injury in\u00a0vitro. Methyl palmitate decreased oxidative stress and enhanced CYP4A14 expression in injured hepatocytes. Molecular docking demonstrated a preferable binding affinity between methyl palmitate and PPAR\u03b1. CETSA and DARTS further validated this interaction by showing that methyl palmitate increased the thermal stability and proteolytic resistance of PPAR\u03b1. Importantly, pharmacological inhibition of PPAR\u03b1 completely abolished the hepatoprotective effects of methyl palmitate in\u00a0vitro. In conclusion, YJSB effectively reduces liver injury in ALD mice. Mechanistically, its active component methyl palmitate activates PPAR\u03b1, thereby upregulating CYP4A14, reshaping arachidonic acid metabolism, and attenuating alcohol\u2010induced oxidative stress in hepatocytes.<\/jats:p>","DOI":"10.1111\/jcmm.71385","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:17:06Z","timestamp":1790741826000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ya\u2010Jie\u2010Sha\u2010Ba Decoction Regulates Arachidonic Acid Metabolism to Treat Alcohol\u2010Associated Liver Disease by Activating PPAR\u03b1\u2013CYP4A14 Axis: Insights From Multi\u2010Omics Analysis and Experimental Validations"],"prefix":"10.1111","volume":"30","author":[{"given":"Dehong","family":"Ma","sequence":"first","affiliation":[{"name":"First School of Clinical Medicine Yunnan University of Chinese Medicine  Kunming China"},{"name":"Xishuangbanna Dai Autonomous Prefecture Dai 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