{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:45:56Z","timestamp":1791996356833,"version":"4.4.0"},"reference-count":39,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The effects and mechanisms of Huangqi Guizhi Wuwu Decoction (HGWD) in cancer cachexia remain unclear. We generated an in\u00a0vivo cachexia model by implanting Lewis lung carcinoma cells into mice and treated animals with HGWD. We evaluated muscle function using grip strength and wire\u2010grip tests. We also assayed markers of muscle synthesis and oxidative stress, and assessed tumour growth to evaluate any antitumor effects of HGWD. Finally, we performed transcriptomic and untargeted metabolomic analyses. HGWD increased muscle strength and restored muscle mass in cachectic mice. HGWD improved levels of proteins associated with muscle anabolism and altered oxidative stress\u2013related factors. Treated mice showed modest tumour inhibition and greater food and water intake. Transcriptomic profiling of gastrocnemius muscle revealed significant enrichment of amino acid\u2013related metabolic pathways after HGWD treatment. Untargeted metabolomics indicated that most branched\u2010chain amino acids were upregulated following HGWD intervention. Further validation showed that HGWD increased both gene and protein expression of adenosylmethionine decarboxylase 1 (AMD1) and spermine oxidase (SMOX), while decreasing gene and protein expression of glycine N\u2010methyltransferase (GNMT) in the gastrocnemius of cachectic mice. The anti\u2010cancer cachexia effect of HGWD appears to be associated with promoting the conversion of S\u2010adenosylmethionine (SAM) to spermidine, thereby correcting amino acid metabolic disturbances.<\/jats:p>","DOI":"10.1111\/jcmm.71387","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T02:50:43Z","timestamp":1790563843000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Huangqi Guizhi Wuwu Decoction Ameliorates Cachexia in Lung Cancer Mice Through Regulating Amino Acid Metabolism: Insights From Transcriptomics, Metabolomics and Experimental Validation"],"prefix":"10.1111","volume":"30","author":[{"given":"Yingjia","family":"Zhou","sequence":"first","affiliation":[{"name":"The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan Integrated Traditional Chinese and Western Medicine Department Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoli","family":"Zhao","sequence":"additional","affiliation":[{"name":"First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmei","family":"Shen","sequence":"additional","affiliation":[{"name":"The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan Integrated Traditional Chinese and Western Medicine Department Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Wu","sequence":"additional","affiliation":[{"name":"The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan Integrated Traditional Chinese and Western Medicine Department Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Li","sequence":"additional","affiliation":[{"name":"The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan Integrated Traditional Chinese and Western Medicine Department Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Liu","sequence":"additional","affiliation":[{"name":"The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan Integrated Traditional Chinese and Western Medicine Department Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3271-4735","authenticated-orcid":false,"given":"Jian","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Kunming Medical University Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Jin","sequence":"additional","affiliation":[{"name":"The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan Integrated Traditional Chinese and Western Medicine Department Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Li","sequence":"additional","affiliation":[{"name":"Science and Technology Achievement Transformation Center of Kunming Medical University Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0820-5436","authenticated-orcid":false,"given":"Huantian","family":"Cui","sequence":"additional","affiliation":[{"name":"First School of Clinical Medicine Yunnan University of Chinese Medicine Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00408\u2010025\u201000801\u2010x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2409515"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.105"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.esmoop.2021.100092"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470\u20102045(10)70218\u20107"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3945\/an.115.011650"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2024.10.017"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587\u2010024\u201000797\u20108"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13519"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina58070966"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.115944"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.914597"},{"key":"e_1_2_11_14_1","first-page":"6752","article-title":"Shashen Maidong Decoction: The Effect of Tnf\u2010Alpha and Il\u20106 on Lung Cancer Cachexia Based on Cancer Toxicity Theory","volume":"13","author":"He M.","year":"2021","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.204989"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.2147\/JPR.S466658"},{"key":"e_1_2_11_17_1","first-page":"446","article-title":"Efficacy and Safety of Huangqi Guizhi Wuwu Decoction for Oxaliplatin\u2010Induced Peripheral Neurotoxicity: A Systematic Review and Meta\u2010Analysis","volume":"30","author":"Yu J.","year":"2024","journal-title":"Alternative Therapies in Health and Medicine"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2025.156950"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13777"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.4103\/jcrt.jcrt_140_24"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045\u2010023\u201001429\u20101"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.926134"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000002257"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389200224666230406114012"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.26599\/FMH.2025.9420041"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12776"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986712803833290"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635589809514677"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.240015"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010\u2010009\u20100186\u20102"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/nan.70025"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241613005"},{"key":"e_1_2_11_33_1","first-page":"3527","article-title":"Differential Reconstitution of Mitochondrial Respiratory Chain Activity and Plasma Redox State by Cysteine and Ornithine in a Model of Cancer Cachexia","volume":"59","author":"Ushmorov A.","year":"1999","journal-title":"Cancer Research"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2007.05.001"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20070651"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13710"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2025.116097"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13797"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26185674"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad1045"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.71387","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.71387","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.71387","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:00:13Z","timestamp":1791993613000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.71387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,27]]},"references-count":39,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/jcmm.71387"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.71387","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,27]]},"assertion":[{"value":"2026-02-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71387"}}