{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:47:25Z","timestamp":1792043245003,"version":"4.4.0"},"reference-count":60,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Sickle cell disease (SCD) is characterized by hemolytic anaemia, painful vaso\u2010occlusive crises, and chronic inflammation resulting partly from platelet activation. It has been shown that platelets express the nucleotide\u2010binding domain leucine\u2010rich repeat containing protein 3 (NLRP3) and the apoptosis\u2010associated speck\u2010like protein containing a CARD (ASC), forming the NLRP3 inflammasome, which controls activation of caspase\u20101 and cleavage of interleukin\u20101\u03b2 (IL\u20101\u03b2). Bruton tyrosine kinase (BTK), known to activate the NLRP3 inflammasome, is also expressed in platelets. In SCD, platelets are activated and can secrete increased quantities of IL\u20101\u03b2, suggesting a possible activation of the NLRP3 inflammasome. Here, we assessed platelet P\u2010selectin expression, caspase\u20101 activation, platelet\u2013neutrophil aggregate formations by flow cytometry and BTK phosphorylation by Western blot in a cohort of 81 adult SCD patients at steady state condition (34 SC and 47 SS patients). We showed an increase in platelet activation in sickle patients and a high level of platelet\u2013neutrophil aggregate in SS patients compared to healthy subjects (17 AA). We also demonstrated platelet caspase\u20101 activation in SCD patients and obtained data suggesting for the first time NLRP3 inflammasome activation in SC patients. Further studies are needed to understand the mechanism of caspase\u20101 activation and evaluate the potential therapeutic efficiency of Ibrutinib, a BTK inhibitor.<\/jats:p>","DOI":"10.1111\/jcmm.71390","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:11:28Z","timestamp":1792041088000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Activation of Platelet Caspase\u20101 and P\u2010Selectin Over\u2010Expression in Sickle Cell Disease Patients: An Involvement of\n                    <scp>NLRP3<\/scp>\n                    Inflammasome?"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6415-3941","authenticated-orcid":false,"given":"Livia","family":"Claude","sequence":"first","affiliation":[{"name":"INSERM, BIGR Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 des Antilles  Paris France"},{"name":"Laboratoire d'H\u00e9matologie CHU de la Guadeloupe  Pointe\u2010\u00e0\u2010Pitre Guadeloupe"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1389-2306","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric","family":"Martino","sequence":"additional","affiliation":[{"name":"INSERM, BIGR Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 des Antilles  Paris France"},{"name":"Service de R\u00e9animation CHU de la Guadeloupe  Pointe\u2010\u00e0\u2010Pitre Guadeloupe"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydiane","family":"Mordier","sequence":"additional","affiliation":[{"name":"INSERM, BIGR Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 des Antilles  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"C\u00e9lanie","sequence":"additional","affiliation":[{"name":"INSERM, BIGR Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 des Antilles  Paris 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Guadeloupe"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9585-4281","authenticated-orcid":false,"given":"Beno\u00eet","family":"Tressi\u00e8res","sequence":"additional","affiliation":[{"name":"Centre d'Investigation Clinique Antilles Guyane, Inserm CIC 1424 CHU de la Guadeloupe  Pointe\u2010\u00e0\u2010Pitre Guadeloupe"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8715-9836","authenticated-orcid":false,"given":"Marc","family":"Romana","sequence":"additional","affiliation":[{"name":"INSERM, BIGR Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 des Antilles  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3913-7664","authenticated-orcid":false,"given":"V\u00e9ronique","family":"Baccini","sequence":"additional","affiliation":[{"name":"INSERM, BIGR Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 des Antilles  Paris 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Guadeloupe"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(10)61029\u2010X"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0969141313507919"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.055202"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2014.114587"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102141.1998.00627.x"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102013\u201011\u2010462432"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200105058"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.06.015"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12084"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/35393"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.atv.20.10.2322"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102004\u201003\u20101108"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0605318"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1400091"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.110.211615"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/09537104.2013.770136"},{"issue":"8","key":"e_1_2_12_18_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1182\/blood.V81.8.2137.2137","article-title":"Thrombospondin 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