{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:46:29Z","timestamp":1791996389391,"version":"4.4.0"},"reference-count":36,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82205143"],"award-info":[{"award-number":["82205143"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Sanse Powder (SSP) has been shown to be effective in the clinical treatment of knee osteoarthritis (KOA), although its active components and underlying mechanisms remain unclear. In this study, we collected the in\u00a0vitro release medium of SSP (SSP\u2010IVRM) using an in\u00a0vitro release test and evaluated its pharmacological effects in a KOA rat model. Ultra\u2010high\u2010performance liquid chromatography\u2013tandem mass spectrometry (UHPLC\u2013MS\/MS) was used to identify active constituents in SSP\u2010IVRM, while network pharmacology and molecular docking were applied to screen key compounds and molecular targets. In\u00a0vivo and in\u00a0vitro experiments using histological staining and molecular biology techniques were performed to investigate the effects of casticin (CAS), a major component of SSP\u2010IVRM, on KOA\u2010related synovitis. UHPLC\u2013MS\/MS identified CAS, monocerin, and chlorogenic acid as major components. Network pharmacology and molecular docking associated these compounds with PI3K signalling and cellular senescence. CAS significantly improved synovial pathology, reduced pro\u2010inflammatory cytokines and chemokines, modulated fibrosis\u2010 and senescence\u2010related genes and proteins, and decreased synovial fibroblast senescence. Transcriptomic analysis further implicated cell adhesion and the PI3K\u2013AKT pathway. Additional experiments demonstrated that CAS alleviates KOA\u2010related inflammation and cellular senescence by inhibiting FAK\/PI3K\/AKT phosphorylation. These findings indicate that CAS in SSP\u2010IVRM reduces synovial fibroblast senescence and inflammation through inhibition of the FAK\/PI3K\/AKT signalling pathway, thereby exerting therapeutic effects against KOA.<\/jats:p>","DOI":"10.1111\/jcmm.71391","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T03:16:35Z","timestamp":1791774995000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Casticin From Sanse Powder\u2010 In\u00a0Vitro Release Medium Inhibits\n                    <scp>FAK<\/scp>\n                    \/\n                    <scp>PI3K<\/scp>\n                    \/\n                    <scp>AKT<\/scp>\n                    Phosphorylation Reducing Synovial Senescence and Alleviates Inflammation in Knee Osteoarthritis"],"prefix":"10.1111","volume":"30","author":[{"given":"Jiangyu","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Orthopedics The Affiliated Hospital of Nanjing University of Chinese Medicine  Nanjing China"},{"name":"The First College of Clinical Medicine Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6409-435X","authenticated-orcid":false,"given":"Peng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Affiliated Hospital of Nanjing University of Chinese Medicine  Nanjing China"},{"name":"The First College of Clinical Medicine Nanjing University of Chinese Medicine  Nanjing China"},{"name":"Jiangsu Provincial Engineering Research Center of TCM External Medication Development and Application  Nanjing China"},{"name":"Nanjing University of Chinese Medicine Clinical Research Institute for Knee Osteoarthritis  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department 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