{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:46:40Z","timestamp":1791996400211,"version":"4.4.0"},"reference-count":35,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005089","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7262095"],"award-info":[{"award-number":["7262095"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["3332024128"],"award-info":[{"award-number":["3332024128"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Age\u2010related hearing loss (ARHL) is a degenerative change in the auditory system, but its underlying mechanism remains unclear. In this study, using RNA sequencing technology, we analysed the expression profiles of mRNAs in the cochlear tissues of ARHL\u2010susceptible C57BL\/6J (C57) mice and relatively resistant CBA\/CaJ (CBA) mice. We found that the mRNA related to stress granules (SGs) was significantly increased in the cochlear tissues of ARHL mice. Combined with current research on the pyroptosis of cochlear hair cells, we constructed an aging model of House Ear Institute\u2010Organ of Corti 1 (HEI\u2010OC1) in\u00a0vitro and found that the expression of SG was upregulated and the expression of pyroptosis\u2010related proteins showed a synergistic upregulation. Furthermore, functional studies involving both G3BP1 knockdown and overexpression suggested that stress granules exert a protective effect by negatively regulating pyroptosis in HEI\u2010OC1 cells. In summary, the results of this study indicate that with aging, the formation of SG increases and the activation of pyroptosis\u2010related markers is enhanced. The dysfunction of G3BP1 further exacerbates these pyroptosis markers, supporting the protective role of SG homeostasis in inhibiting pyroptosis activation under stress conditions. Preliminary verification suggests that the SG\u2010pyroptosis axis may be a potential target for regulating the pyroptosis of cochlear hair cells.<\/jats:p>","DOI":"10.1111\/jcmm.71403","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T02:46:02Z","timestamp":1791773162000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomics Implicates the\n                    <scp>G3BP1<\/scp>\n                    \u2013\n                    <scp>SG<\/scp>\n                    Axis in Cochlear Hair\u2010Cell Pyroptosis During Age\u2010Related Hearing Loss"],"prefix":"10.1111","volume":"30","author":[{"given":"Chen","family":"Li","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Eight\u2010Year Medical Doctor Program Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Clinical Biobank (ISO 20387) Peking Union Medical College Hospital, Chinese Academy of Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyao","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Huo","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0153-582X","authenticated-orcid":false,"given":"Hua","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,11]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2024.1428564"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cger.2018.01.001"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archger.2025.105847"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/00034894931020S101"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.24476"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2023.1308823"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3934\/molsci.2016.3.300"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-05634-1"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-025-04880-y"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2017.08.015"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2024.111964"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00608"},{"key":"e_1_2_12_14_1","unstructured":"M. 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