{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:06:30Z","timestamp":1791986790362,"version":"4.4.0"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,9,24]],"date-time":"2012-09-24T00:00:00Z","timestamp":1348444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key Basic Research Program of China","award":["No.2007CB947304"],"award-info":[{"award-number":["No.2007CB947304"]}]},{"name":"National Key Basic Research Program of China","award":["No.2010CB944801"],"award-info":[{"award-number":["No.2010CB944801"]}]},{"name":"Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality","award":["PHR20090510"],"award-info":[{"award-number":["PHR20090510"]}]},{"name":"Funding Project to Science Facility in Institutions of Higher Learning Under the jurisdiction of Beijing Municipality","award":["PXM 2009-014226-074691"],"award-info":[{"award-number":["PXM 2009-014226-074691"]}]},{"name":"Funding Project to Science Facility in Institutions of Higher Learning Under the jurisdiction of Beijing Municipality","award":["PXM2011-014226-07-000066"],"award-info":[{"award-number":["PXM2011-014226-07-000066"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The purpose of this study was to investigate the immunomodulatory properties of periodontal ligament stem cells derived from inflamed periodontal ligament tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Periodontal ligament stem cells were identified and isolated from healthy or inflamed periodontal ligament tissues. Peripheral blood mononuclear cells were cocultured with inflamed or healthy periodontal ligament stem cells, and T\u2010lymphocyte proliferation was determined by incubation with carboxyfluorescein succinimidyl ester. T\u2010helper cells (Th1\/Th2, Th17) and regulatory T cells were analysed by flow cytometry. Cytokine profiles in supernatants were tested with a cytometric bead array.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to healthy cells, inflamed periodontal ligament stem cells showed significantly diminished inhibition of T\u2010cell proliferation. In cocultures, stimulated peripheral blood mononuclear cells showed significantly less induction of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4+<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25+<jats:styled-content style=\"fixed-case\">FOXP<\/jats:styled-content>3+ regulatory T cells and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 secretion in the presence of inflamed compared with healthy periodontal ligament stem cells. Furthermore, suppression of Th17 differentiation and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017 production by inflamed periodontal ligament stem cells was significantly lesser than by healthy cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrated that inflamed periodontal ligament stem cells had markedly dysfunctional immunomodulatory properties; this may contribute to an imbalanced immune response, acceleration of osteoclastogenesis and inflammatory alveolar bone loss in periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12009","type":"journal-article","created":{"date-parts":[[2012,8,24]],"date-time":"2012-08-24T15:33:18Z","timestamp":1345822398000},"page":"1174-1182","source":"Crossref","is-referenced-by-count":147,"title":["Mesenchymal stem cells derived from inflamed periodontal ligaments exhibit impaired immunomodulation"],"prefix":"10.1111","volume":"39","author":[{"given":"Dayong","family":"Liu","sequence":"first","affiliation":[{"name":"Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction Capital Medical University School of Stomatology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junji","family":"Xu","sequence":"additional","affiliation":[{"name":"Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction Capital Medical University School of Stomatology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ousheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction Capital Medical University School of Stomatology Beijing 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