{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:39:01Z","timestamp":1790026741006,"version":"4.0.1"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,11,22]],"date-time":"2012-11-22T00:00:00Z","timestamp":1353542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Saliva is a useful diagnostic fluid for oral\u2010related diseases. Monitoring salivary biomarkers for oral and systemic diseases could become an important complement to clinical examinations in epidemiological surveys. Recent findings indicate that it is possible to detect biomarkers for oral diseases within saliva samples. The aim of this study was to investigate if known salivary biomarkers could be used for epidemiological studies for detection of periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>A randomly selected sample of adults (20\u201389\u00a0years) living in Southern Sweden were invited to participate. Four hundred and fifty\u2010one individuals were examined clinically using standard examination procedures. Stimulated saliva samples were collected and analysed for concentrations of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2, \u20106, \u20108, lysozyme, matrix metalloproteinases (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>)\u20108 and tissue inhibitor of metalloproteinase (<jats:styled-content style=\"fixed-case\">TIMP<\/jats:styled-content>)\u20101 using <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, immunofluorometric assay or Luminex assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Patients with severe periodontitis presented with elevated salivary concentrations of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). In addition, the <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108\/<jats:styled-content style=\"fixed-case\">TIMP<\/jats:styled-content>\u20101 ratio was significantly higher in the severe periodontitis group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). Smokers compared with non\u2010smokers showed slightly lower concentrations of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20138 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.052).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This investigation shows that <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2, <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 and the ratio of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108\/<jats:styled-content style=\"fixed-case\">TIMP<\/jats:styled-content>\u20101 could be used as markers of periodontal disease in larger patient populations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12038","type":"journal-article","created":{"date-parts":[[2012,10,30]],"date-time":"2012-10-30T07:36:42Z","timestamp":1351582602000},"page":"140-147","source":"Crossref","is-referenced-by-count":151,"title":["Salivary biomarkers of oral health \u2013 a cross\u2010sectional study"],"prefix":"10.1111","volume":"40","author":[{"given":"Nilminie","family":"Rathnayake","sequence":"first","affiliation":[{"name":"Division of Periodontology Department of Dental Medicine Karolinska Institutet Huddinge Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigvard","family":"\u00c5kerman","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnostics Malm\u00f6 University Faculty of Odontology Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bjorn","family":"Klinge","sequence":"additional","affiliation":[{"name":"Division of Periodontology Department of Dental Medicine Karolinska Institutet Huddinge Sweden"},{"name":"Department of Periodontology Malm\u00f6 University Faculty of Odontology Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Lundegren","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnostics Malm\u00f6 University Faculty of Odontology Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Jansson","sequence":"additional","affiliation":[{"name":"Department of Periodontology Malm\u00f6 University Faculty of Odontology Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ylva","family":"Tryselius","sequence":"additional","affiliation":[{"name":"Division of Periodontology Department of Dental Medicine Karolinska Institutet Huddinge Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Sorsa","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases University of Helsinki Helsinki University Central Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Gustafsson","sequence":"additional","affiliation":[{"name":"Division of Periodontology Department of Dental Medicine Karolinska Institutet Huddinge Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,11,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.11-S.2066"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2012.110616"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(05)80092-3"},{"key":"e_1_2_6_6_1","first-page":"107","article-title":"The relationship between diabetes mellitus and destructive periodontal disease: a meta\u2010analysis","volume":"7","author":"Ch\u00e1varry N. 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