{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T18:06:59Z","timestamp":1789927619376,"version":"4.0.1"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,4,7]],"date-time":"2013-04-07T00:00:00Z","timestamp":1365292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012819","name":"Egyptian Cultural and Educational Bureau","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100012819","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Test whether human periodontal ligament fibroblasts (<jats:styled-content style=\"fixed-case\">PDLF<\/jats:styled-content>s) retain homeostatic responses to a physiological compressive force during chronic periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Six cell lines were established from periodontally healthy individuals (H\u2010<jats:styled-content style=\"fixed-case\">PDLF<\/jats:styled-content>s) and another six were cultured from patients diagnosed with chronic periodontitis (D\u2010<jats:styled-content style=\"fixed-case\">PDLF<\/jats:styled-content>s). Compressive force at 150\u00a0psi was applied to H\u2010and D\u2010<jats:styled-content style=\"fixed-case\">PDLF<\/jats:styled-content>s for 3\u00a0h on 2 consecutive days. After compression, comparisons between H\u2010and D\u2010<jats:styled-content style=\"fixed-case\">PDLF<\/jats:styled-content>s were performed by gene expression analysis of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, proteases and 84 inflammation\u2010related targets using real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compression of H\u2010PDLFs resulted in a significant increase only in <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20101 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>. In contrast, the same compressive force on D\u2010PDLFs produced significant increases in the expression of MMPs\u20101,\u20107,\u20109 and \u201016. Moreover, compression of H\u2010PDLFs resulted in down\u2010regulation of IL\u20106, while IL\u20106 was significantly up\u2010regulated in compressed D\u2010PDLFs. Compression of H\u2010PDLFs slightly up\u2010regulated 3 and significantly down\u2010regulated 15 inflammation\u2010related genes, while the same treatment strongly up\u2010regulated 21 inflammation\u2010related genes in D\u2010PDLFs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest a fundamental difference in the inflammatory response of healthy versus diseased <jats:styled-content style=\"fixed-case\">PDLF<\/jats:styled-content>s under physiological compression. Maintenance of these characteristics in vitro suggests that these cells may be at least partly responsible for the persistence of inflammation and localized susceptibility in chronic periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12100","type":"journal-article","created":{"date-parts":[[2013,2,23]],"date-time":"2013-02-23T17:07:44Z","timestamp":1361639264000},"page":"661-671","source":"Crossref","is-referenced-by-count":21,"title":["Human periodontal ligament fibroblast responses to compression in chronic periodontitis"],"prefix":"10.1111","volume":"40","author":[{"given":"Ahmed R.","family":"El\u2010Awady","sequence":"first","affiliation":[{"name":"Department of Periodontology School of Dentistry Al\u2010Azhar University Cairo Egypt"},{"name":"Department of Oral Biology Georgia Health Sciences University Augusta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol A.","family":"Lapp","sequence":"additional","affiliation":[{"name":"Department of Oral Biology Georgia Health Sciences University Augusta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed Y.","family":"Gamal","sequence":"additional","affiliation":[{"name":"Department of Periodontology School of Dentistry Al\u2010Azhar University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed M.","family":"Sharawy","sequence":"additional","affiliation":[{"name":"Department of Oral Biology Georgia Health Sciences University Augusta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl H.","family":"Wenger","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Georgia Health Sciences University Augusta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher W.","family":"Cutler","sequence":"additional","affiliation":[{"name":"Department of Periodontology Georgia Health Sciences University Augusta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina L. 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