{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T04:25:29Z","timestamp":1780633529817,"version":"3.54.1"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,6,7]],"date-time":"2013-06-07T00:00:00Z","timestamp":1370563200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"University of Michigan School of Dentistry"},{"name":"MUSC Center for Oral Health Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Following toll\u2010like receptor (<jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>) engagement, lipopolysaccharide (<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>) can stimulate the expression of pro\u2010inflammatory cytokines thus activating the innate immune response. The production of inflammatory cytokines results, in part, from the activation of kinase\u2010induced signalling cascades and transcriptional factors. Of the four distinct classes of mitogen\u2010activated protein kinases (<jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>) described in mammals, p38, c\u2010Jun N\u2010terminal activated kinases (<jats:styled-content style=\"fixed-case\">JNK<\/jats:styled-content>1\u20103) and extracellular activated kinases (<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>1,2) are the best studied. Previous data have established that p38 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> signalling is required for inflammation and bone loss in periodontal disease pre\u2010clinical animal models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials &amp; Methods<\/jats:title><jats:p>In this study, we obtained healthy and diseased periodontal tissues along with clinical parameters and microbiological parameters. Excised fixed tissues were immunostained with total and phospho\u2010specific antibodies against p38, <jats:styled-content style=\"fixed-case\">JNK<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content> kinases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Intensity scoring from immunostained tissues was correlated with clinical periodontal parameters. Rank correlations with clinical indices were statistically significantly positive (<jats:italic>p<\/jats:italic>\u2010value &lt; 0.05) for total p38 (correlations ranging 0.49\u20130.68), phospho\u2010p38 (range 0.44\u20130.56), and total <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content> (range 0.52\u20130.59) levels, and correlations with <jats:styled-content style=\"fixed-case\">JNK<\/jats:styled-content> levels also supported association (range 0.42\u20130.59). Phospho\u2010<jats:styled-content style=\"fixed-case\">JNK<\/jats:styled-content> and phospho\u2010<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content> showed no significant positive correlation with clinical parameters of disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These data strongly implicate p38 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> as a major <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> involved in human periodontal inflammation and severity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12123","type":"journal-article","created":{"date-parts":[[2013,5,27]],"date-time":"2013-05-27T05:06:38Z","timestamp":1369631198000},"page":"757-764","source":"Crossref","is-referenced-by-count":25,"title":["Differential expression of mitogen activating protein kinases in periodontitis"],"prefix":"10.1111","volume":"40","author":[{"given":"Suncica","family":"Travan","sequence":"first","affiliation":[{"name":"Department of Periodontics and Oral Medicine School of Dentistry University of Michigan Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Oral Medicine School of Dentistry University of Michigan Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisha J.","family":"D'Silva","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Oral Medicine School of Dentistry University of Michigan Ann Arbor MI USA"},{"name":"Department of Pathology Medical School University of Michigan Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth H.","family":"Slate","sequence":"additional","affiliation":[{"name":"Department of Statistics Florida State University Tallahassee FL USA"},{"name":"Department of Craniofacial Biology and Center for Oral Health Research Medical University of South Carolina Charleston SC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith L.","family":"Kirkwood","sequence":"additional","affiliation":[{"name":"Department of Craniofacial Biology and Center for Oral Health Research Medical University of South Carolina Charleston SC USA"},{"name":"Department of Microbiology and Immunology Medical University of South Carolina Charleston SC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,6,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.7.1148"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.4049\/jimmunol.160.1.403","article-title":"IL\u20101 and TNF antagonists inhibit the inflammatory response and bone loss in experimental periodontitis","volume":"160","author":"Assuma R.","year":"1998","journal-title":"The Journal of Immunology"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2008.00448.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1999.0979"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M513646200"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.2.196"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5929"},{"key":"e_1_2_6_9_1","first-page":"507","article-title":"Frequency distribution of the values of the correlation coefficient in samples of an indefinitely large population","volume":"10","author":"Fisher R. 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