{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:08:40Z","timestamp":1791644920849,"version":"4.3.4"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,12,12]],"date-time":"2013-12-12T00:00:00Z","timestamp":1386806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["U01-DE014961"],"award-info":[{"award-number":["U01-DE014961"]}]},{"DOI":"10.13039\/100000097","name":"NCRR","doi-asserted-by":"crossref","award":["ULRR0000042"],"award-info":[{"award-number":["ULRR0000042"]}],"id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Swiss Society of Periodontology"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Assess the ability of a panel of gingival crevicular fluid (<jats:styled-content style=\"fixed-case\">GCF<\/jats:styled-content>) biomarkers as predictors of periodontal disease progression (<jats:styled-content style=\"fixed-case\">PDP<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>In this study, 100 individuals participated in a 12\u2010month longitudinal investigation and were categorized into four groups according to their periodontal status. <jats:styled-content style=\"fixed-case\">GCF<\/jats:styled-content>, clinical parameters and saliva were collected bi\u2010monthly. Subgingival plaque and serum were collected bi\u2010annually. For 6\u00a0months, no periodontal treatment was provided. At 6\u00a0months, patients received periodontal therapy and continued participation from 6 to 12\u00a0months. <jats:styled-content style=\"fixed-case\">GCF<\/jats:styled-content> samples were analysed by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> for <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108, <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109, Osteoprotegerin, C\u2010reactive Protein and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101<jats:italic>\u03b2<\/jats:italic>. Differences in median levels of <jats:styled-content style=\"fixed-case\">GCF<\/jats:styled-content> biomarkers were compared between stable and progressing participants using Wilcoxon Rank Sum test (<jats:italic>p\u00a0<\/jats:italic>=\u00a00.05). Clustering algorithm was used to evaluate the ability of oral biomarkers to classify patients as either stable or progressing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eighty\u2010three individuals completed the 6\u2010month monitoring phase. With the exception of <jats:styled-content style=\"fixed-case\">GCF<\/jats:styled-content> C\u2010reactive protein, all biomarkers were significantly higher in the <jats:styled-content style=\"fixed-case\">PDP<\/jats:styled-content> group compared to stable patients. Clustering analysis showed highest sensitivity levels when biofilm pathogens and <jats:styled-content style=\"fixed-case\">GCF<\/jats:styled-content> biomarkers were combined with clinical measures, 74% (95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\u00a0=\u00a061, 86).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Signature of <jats:styled-content style=\"fixed-case\">GCF<\/jats:styled-content> fluid\u2010derived biomarkers combined with pathogens and clinical measures provides a sensitive measure for discrimination of <jats:styled-content style=\"fixed-case\">PDP<\/jats:styled-content> (ClinicalTrials.gov <jats:styled-content style=\"fixed-case\">NCT<\/jats:styled-content>00277745).<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12194","type":"journal-article","created":{"date-parts":[[2013,11,7]],"date-time":"2013-11-07T11:36:19Z","timestamp":1383824179000},"page":"113-120","source":"Crossref","is-referenced-by-count":204,"title":["Crevicular fluid biomarkers and periodontal disease progression"],"prefix":"10.1111","volume":"41","author":[{"given":"Janet S.","family":"Kinney","sequence":"first","affiliation":[{"name":"Department of Periodontics and Oral Medicine &amp; Michigan Center for Oral Health Research University of Michigan School of Dentistry Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago","family":"Morelli","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Oral Medicine &amp; Michigan Center for Oral Health Research University of Michigan School of Dentistry Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Oral Medicine &amp; Michigan Center for Oral Health Research University of Michigan School of Dentistry Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Braun","sequence":"additional","affiliation":[{"name":"Department of Biostatistics School of Public Health University of Michigan Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph A.","family":"Ramseier","sequence":"additional","affiliation":[{"name":"Department of Periodontology School of Dental Medicine University of Bern Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim V.","family":"Sugai","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Oral Medicine &amp; Michigan Center for Oral Health Research University of Michigan School of Dentistry Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William V.","family":"Giannobile","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Oral Medicine &amp; Michigan Center for Oral Health Research University of Michigan School of Dentistry Ann Arbor MI USA"},{"name":"Department of Biomedical Engineering College of Engineering University of Michigan Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2011.01805.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2012.110616"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2009.00544.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2011.01817.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1984.tb01331.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1982.tb02108.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01563.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1983.tb01278.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1991.tb01700.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511399908"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1983.tb01292.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1991.62.6.377"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1988.tb01592.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1997.tb00474.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.1999.260607.x"},{"key":"e_1_2_6_18_1","first-page":"25","article-title":"The salivary secretions in health and disease","volume":"8","author":"Mandel I. 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