{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T17:45:13Z","timestamp":1778694313780,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,2,10]],"date-time":"2014-02-10T00:00:00Z","timestamp":1391990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["07\/55291-9"],"award-info":[{"award-number":["07\/55291-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To evaluate the changes occurring in the subgingival microbial profile of subjects with generalized chronic periodontitis (ChP) treated by scaling and root planing (<jats:styled-content style=\"fixed-case\">SRP<\/jats:styled-content>) alone or with metronidazole (<jats:styled-content style=\"fixed-case\">MTZ<\/jats:styled-content>) or <jats:styled-content style=\"fixed-case\">MTZ<\/jats:styled-content>\u00a0+\u00a0amoxicillin (<jats:styled-content style=\"fixed-case\">AMX<\/jats:styled-content>). A secondary aim was to examine a possible added effect of chlorhexidine (<jats:styled-content style=\"fixed-case\">CHX<\/jats:styled-content>) to these therapies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>One hundred and eighteen subjects were randomly assigned to receive <jats:styled-content style=\"fixed-case\">SRP<\/jats:styled-content>\u2010only or with <jats:styled-content style=\"fixed-case\">MTZ<\/jats:styled-content> [400\u00a0mg\/thrice a day (<jats:styled-content style=\"fixed-case\">TID<\/jats:styled-content>)] or <jats:styled-content style=\"fixed-case\">MTZ<\/jats:styled-content>\u00a0+\u00a0<jats:styled-content style=\"fixed-case\">AMX<\/jats:styled-content> (500\u00a0mg\/<jats:styled-content style=\"fixed-case\">TID<\/jats:styled-content>) for 14\u00a0days. Half of the subjects in each group rinsed with 0.12% CHX twice a day (<jats:styled-content style=\"fixed-case\">BID<\/jats:styled-content>) for 2\u00a0months. Nine subgingival plaque samples\/subject were analysed by checkerboard <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> hybridization for 40 bacterial species at baseline, 3, 6 and 12\u00a0months post\u2010therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At 12\u00a0months, the antibiotic\u2010treated groups harboured lower mean counts and proportions of key periodontal pathogens than the <jats:styled-content style=\"fixed-case\">SRP<\/jats:styled-content> group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). These benefits were observed at initially deep and shallow sites. Initial reductions in periodontal pathogens obtained with <jats:styled-content style=\"fixed-case\">SRP<\/jats:styled-content> partially rebound after 12\u00a0months. <jats:styled-content style=\"fixed-case\">CHX<\/jats:styled-content> rinsing enhanced the microbiological effects of the <jats:styled-content style=\"fixed-case\">MTZ<\/jats:styled-content>\u00a0+\u00a0<jats:styled-content style=\"fixed-case\">AMX<\/jats:styled-content> treatment in shallow sites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The adjunctive use of <jats:styled-content style=\"fixed-case\">MTZ<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MTZ<\/jats:styled-content>\u00a0+\u00a0<jats:styled-content style=\"fixed-case\">AMX<\/jats:styled-content> results in a greater reduction in the levels of periodontal pathogens in generalized ChP subjects compared to <jats:styled-content style=\"fixed-case\">SRP<\/jats:styled-content> alone.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12217","type":"journal-article","created":{"date-parts":[[2013,12,19]],"date-time":"2013-12-19T11:35:00Z","timestamp":1387452900000},"page":"366-376","source":"Crossref","is-referenced-by-count":60,"title":["Metronidazole alone or with amoxicillin as adjuncts to non\u2010surgical treatment of chronic periodontitis: a secondary analysis of microbiological results from a randomized clinical trial"],"prefix":"10.1111","volume":"41","author":[{"given":"Geisla M. S.","family":"Soares","sequence":"first","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana A. V.","family":"Mendes","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maike P.","family":"Silva","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo","family":"Faveri","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Teles","sequence":"additional","affiliation":[{"name":"Department of Applied Oral Sciences The Forsyth Institute Cambridge MA USA"},{"name":"Department of Oral Medicine, Infection and Immunity Harvard School of Dental Medicine Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sigmund S.","family":"Socransky","sequence":"additional","affiliation":[{"name":"Department of Applied Oral Sciences The Forsyth Institute Cambridge MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoshan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Applied Oral Sciences The Forsyth Institute Cambridge MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciene C.","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magda","family":"Feres","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,2,10]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.174"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_5_4_1","first-page":"1165","article-title":"The control of the false discovery rate in multiple testing under dependency","volume":"4","author":"Benjamini Y.","year":"2001","journal-title":"The Annals of Statistics"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02455.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00720.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.090390"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.5.749"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2001.028007597.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12004"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2007.00403.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2006.01040.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2873"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.10.006"},{"key":"e_1_2_5_15_1","first-page":"7","article-title":"Periodontal risk assessment (PRA) for patients in supportive periodontal therapy (SPT)","volume":"1","author":"Lang N. 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