{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:08:59Z","timestamp":1791644939149,"version":"4.3.4"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,2,17]],"date-time":"2014-02-17T00:00:00Z","timestamp":1392595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Fund for Scientific and Technological Development, Chilean Government","award":["1120138"],"award-info":[{"award-number":["1120138"]}]},{"DOI":"10.13039\/501100005634","name":"Finnish Dental Society Apollonia","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005634","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Research Foundation of Helsinki University Central Hospital"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2014,4]]},"abstract":"Abstract<\/jats:title>\n \n Aim<\/jats:title>\n \n To identify the diagnostic accuracy of gingival crevicular fluid (\n GCF<\/jats:styled-content>\n ) candidate biomarkers to discriminate periodontitis from the inflamed and healthy sites, and to compare the performance of two independent matrix metalloproteinase (\n MMP<\/jats:styled-content>\n )\u20108 immunoassays.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n Cross sectional study<\/jats:italic>\n . GCF (\n N<\/jats:italic>\n \u00a0=\u00a058 sites) was collected from healthy, gingivitis and chronic periodontitis volunteers and analysed for levels of azurocidin, chemokine ligand 5,\n MPO<\/jats:styled-content>\n ,\n TIMP<\/jats:styled-content>\n \u20101\n MMP<\/jats:styled-content>\n \u201013 and\n MMP<\/jats:styled-content>\n \u201014 by\n ELISA<\/jats:styled-content>\n or activity assays.\n MMP<\/jats:styled-content>\n \u20108 was assayed by immunofluorometric assay (\n IFMA<\/jats:styled-content>\n ) and\n ELISA<\/jats:styled-content>\n . Statistical analysis was performed using linear mixed\u2010effects models and Bayesian statistics in R and Stata V11.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n MMP<\/jats:styled-content>\n \u20108,\n MPO<\/jats:styled-content>\n , azurocidin and total\n MMP<\/jats:styled-content>\n \u201013 and\n MMP<\/jats:styled-content>\n \u201014 were higher in periodontitis compared to gingivitis and healthy sites (\n p<\/jats:italic>\n \u00a0<\u00a00.05). A very high correlation between\n MPO<\/jats:styled-content>\n and\n MMP<\/jats:styled-content>\n \u20108 was evident in the periodontitis group (\n r<\/jats:italic>\n \u00a0=\u00a00.95,\n p<\/jats:italic>\n \u00a0<\u00a00.0001).\n MPO<\/jats:styled-content>\n , azurocidin and total levels of\n MMP<\/jats:styled-content>\n \u20108,\n MMP<\/jats:styled-content>\n \u201013 and\n MMP<\/jats:styled-content>\n \u201014 showed high diagnostic accuracy (\u22650.90), but only\n MMP<\/jats:styled-content>\n \u20108 and\n MPO<\/jats:styled-content>\n were significantly higher in the periodontitis\n versus<\/jats:italic>\n gingivitis sites.\n MMP<\/jats:styled-content>\n \u20108 determined by\n IFMA<\/jats:styled-content>\n correlated more strongly with periodontal status and showed higher diagnostic accuracy than\n ELISA<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n MPO<\/jats:styled-content>\n and collagenolytic\n MMP<\/jats:styled-content>\n s are highly discriminatory biomarkers for site\u2010specific diagnosis of periodontitis. The comparison of two quantitative\n MMP<\/jats:styled-content>\n \u20108 methods demonstrated\n IFMA<\/jats:styled-content>\n to be more accurate than\n ELISA<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jcpe.12223","type":"journal-article","created":{"date-parts":[[2013,12,30]],"date-time":"2013-12-30T09:24:40Z","timestamp":1388395480000},"page":"348-356","source":"Crossref","is-referenced-by-count":118,"title":["Matrix metalloproteinases and myeloperoxidase in gingival crevicular fluid provide site\u2010specific diagnostic value for chronic periodontitis"],"prefix":"10.1111","volume":"41","author":[{"given":"Jussi M.","family":"Leppilahti","sequence":"first","affiliation":[{"name":"Institute of Dentistry University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A.","family":"Hern\u00e1ndez\u2010R\u00edos","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry Faculty of Dentistry University of Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge A.","family":"Gamonal","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry Faculty of Dentistry University of Chile Santiago Chile"},{"name":"Laboratory of Periodontal Biology Faculty of Dentistry University of Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taina","family":"Tervahartiala","sequence":"additional","affiliation":[{"name":"Institute of Dentistry University of Helsinki Helsinki Finland"},{"name":"Department of Oral and Maxillofacial Diseases Helsinki University Central Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romina","family":"Brignardello\u2010Petersen","sequence":"additional","affiliation":[{"name":"Department of Pathology Faculty of Dentistry University of Chile Santiago Chile"},{"name":"Institute of Health Policy Management and Evaluation University of Toronto Toronto Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paivi","family":"Mantyla","sequence":"additional","affiliation":[{"name":"Institute of Dentistry University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Sorsa","sequence":"additional","affiliation":[{"name":"Institute of Dentistry University of Helsinki Helsinki Finland"},{"name":"Department of Oral and Maxillofacial Diseases Helsinki University Central Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Laboratory of Periodontal Biology Faculty of Dentistry University of Chile Santiago Chile"},{"name":"Department of Pathology Faculty of Dentistry University of Chile Santiago 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