{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T10:55:32Z","timestamp":1770980132514,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,6,9]],"date-time":"2014-06-09T00:00:00Z","timestamp":1402272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Medtronic Spine & Biologics, Memphis, TN, USA"},{"name":"Nobel Biocare AG"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Application of recombinant human bone morphogenetic protein\u20102 (rh<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20102) has been associated with significant adverse events in craniofacial settings, including swelling and seroma formation. Recent work has demonstrated an inverse relationship between bone formation\/maturation and rh<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20102 dose, frequency\/severity of adverse events increasing with rising dose.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The objective of this study was to determine the most effective dose for rh<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20102 soak\u2010loaded onto an absorbable collagen sponge (<jats:styled-content style=\"fixed-case\">ACS<\/jats:styled-content>) carrier for bone formation\/maturation using an established defect model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>One hundred sixty\u2010eight outbred male Sprague\u2010Dawley rats, age 11\u201313\u00a0weeks, weight 325\u2013375\u00a0g randomized into seven groups of 24 subdivided into groups of eight, were used to provide radiographic and light microscopy observations of bone formation\/maturation and aberrant healing events at 2, 4 and 8\u00a0weeks following application of rh<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20102\/<jats:styled-content style=\"fixed-case\">ACS<\/jats:styled-content> into critical\u2010size, \u00f88\u2010mm, through\u2010through, calvarial osteotomy defects for a dose of 1.25, 2.5, 5.0, 10.0 and 20.0\u00a0<jats:italic>\u03bc<\/jats:italic>g rh<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20102\/defect, or serve as <jats:styled-content style=\"fixed-case\">ACS<\/jats:styled-content> or sham\u2010surgery controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>rh<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20102 dosages \u22652.5\u00a0<jats:italic>\u03bc<\/jats:italic>g\/defect showed histological defect closure &gt;90% within 2\u00a0weeks, and complete resolution within 4\u00a0weeks. Adverse healing events including swelling, excessive bone formation or seroma formation could not be determined with certainty in this defect model. Notably <jats:styled-content style=\"fixed-case\">ACS<\/jats:styled-content> control sites showed complete defect closure at the 8\u2010week healing interval.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>rh<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20102\/<jats:styled-content style=\"fixed-case\">ACS<\/jats:styled-content> accelerates local bone formation in the rat critical\u2010size through\u2010through calvarial defect model once reaching an osteoinductive dose threshold. This threshold may already be reached at a 1.25\u2010\/2.5\u2010<jats:italic>\u03bc<\/jats:italic>g dose in this model. No further enhancement to bone formation\/maturation may be observed adding rh<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20102 above the 2.5\u2010<jats:italic>\u03bc<\/jats:italic>g dose. The 1.25\u201320.0\u00a0<jats:italic>\u03bc<\/jats:italic>g dose range did not invoke appreciable aberrant healing events.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12270","type":"journal-article","created":{"date-parts":[[2014,5,7]],"date-time":"2014-05-07T18:30:28Z","timestamp":1399487428000},"page":"827-836","source":"Crossref","is-referenced-by-count":56,"title":["Effect of rh<scp>BMP<\/scp>\u20102 dose on bone formation\/maturation in a rat critical\u2010size calvarial defect model"],"prefix":"10.1111","volume":"41","author":[{"given":"Manuel","family":"Pelaez","sequence":"first","affiliation":[{"name":"Laboratory for Applied Periodontal &amp; Craniofacial Regeneration (LAPCR) Georgia Regents University College of Dental Medicine Augusta GA USA"},{"name":"US Army Dental Activity Fort Bragg NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiano","family":"Susin","sequence":"additional","affiliation":[{"name":"Laboratory for Applied Periodontal &amp; Craniofacial Regeneration (LAPCR) Georgia Regents University College of Dental Medicine Augusta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaebum","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory for Applied Periodontal &amp; Craniofacial Regeneration (LAPCR) Georgia Regents University College of Dental Medicine Augusta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiago","family":"Fiorini","sequence":"additional","affiliation":[{"name":"Laboratory for Applied Periodontal &amp; Craniofacial Regeneration (LAPCR) Georgia Regents University College of Dental Medicine Augusta GA USA"},{"name":"Section of Periodontology School of Dentistry Federal University Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederick C.","family":"Bisch","sequence":"additional","affiliation":[{"name":"US Army Advanced Education Program in Periodontics Fort Gordon GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas R.","family":"Dixon","sequence":"additional","affiliation":[{"name":"US Army Dental Activity West Point NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"James C.","family":"McPherson","sequence":"additional","affiliation":[{"name":"US Army Department of Clinical Investigations Ft. Gordon GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda N.","family":"Buxton","sequence":"additional","affiliation":[{"name":"Medtronic Spine &amp; Biologics Memphis TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulf M.E.","family":"Wikesj\u00f6","sequence":"additional","affiliation":[{"name":"Laboratory for Applied Periodontal &amp; Craniofacial Regeneration (LAPCR) Georgia Regents University College of Dental Medicine Augusta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,6,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.6.787"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.K.00469"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2011.01428.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2010.40.1.11"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1993.64.9.878"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cid.12156"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12148"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.12.2216"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2013.0442"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.02.045"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2006.01324.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050268"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.01.075"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2008.01567.x"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00264-007-0418-6"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2009.0658"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.9.1526"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050328"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00772.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.8.1287"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0501.1997.080503.x"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2012.113"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12001"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2002.00021.x"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.150.3698.893"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-200202000-00011"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coms.2007.07.004"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2008.01321.x"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1708-8208.2003.tb00192.x"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2004.00971.x"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2008.01318.x"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3201241"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01547.x"}],"container-title":["Journal of Clinical Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcpe.12270","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcpe.12270","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.12270","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T01:51:41Z","timestamp":1696211501000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcpe.12270"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,9]]},"references-count":33,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1111\/jcpe.12270"],"URL":"https:\/\/doi.org\/10.1111\/jcpe.12270","archive":["Portico"],"relation":{},"ISSN":["0303-6979","1600-051X"],"issn-type":[{"value":"0303-6979","type":"print"},{"value":"1600-051X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,9]]}}}