{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:39:36Z","timestamp":1787161176319,"version":"build-2736575974"},"reference-count":73,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,1,8]],"date-time":"2015-01-08T00:00:00Z","timestamp":1420675200000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Birmingham"},{"DOI":"10.13039\/100013956","name":"Black Country Comprehensive Local Research Network","doi-asserted-by":"crossref","award":["10318"],"award-info":[{"award-number":["10318"]}],"id":[{"id":"10.13039\/100013956","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Medical Research Council"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the chemotactic accuracy of peripheral blood neutrophils from patients with chronic periodontitis compared with matched healthy controls, before and after non\u2010surgical periodontal therapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material &amp; Methods<\/jats:title>\n                    <jats:p>\n                      Neutrophils were isolated from patients and controls (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a018) by density centrifugation. Using the Insall chamber and video microscopy, neutrophils were analysed for directional chemotaxis towards\n                      <jats:italic>N<\/jats:italic>\n                      \u2010formyl\u2010methionyl\u2010leucyl\u2010phenylalanine [fMLP (10\u00a0nM), or CXCL8 (200\u00a0ng\/ml)]. Circular statistics were utilized for the analysis of cell movement.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Prior to treatment, neutrophils from patients with chronic periodontitis had significantly reduced speed, velocity and chemotactic accuracy compared to healthy controls for both chemoattractants. Following periodontal treatment, patient neutrophils continued to display reduced speed in response to both chemoattractants. However, velocity and accuracy were normalized for the weak chemoattractant CXCL8 while they remained significantly reduced for fMLP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Chronic periodontitis is associated with reduced neutrophil chemotaxis, and this is only partially restored by successful treatment. Dysfunctional neutrophil chemotaxis may predispose patients with periodontitis to their disease by increasing tissue transit times, thus exacerbating neutrophil\u2010mediated collateral host tissue damage.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.12326","type":"journal-article","created":{"date-parts":[[2014,10,31]],"date-time":"2014-10-31T11:47:52Z","timestamp":1414756072000},"page":"1-11","source":"Crossref","is-referenced-by-count":70,"title":["Impaired neutrophil directional chemotactic accuracy in chronic periodontitis patients"],"prefix":"10.1111","volume":"42","author":[{"given":"Helen M.","family":"Roberts","sequence":"first","affiliation":[{"name":"Periodontal Research Group and MRC Centre for Immune Regulation University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin R.","family":"Ling","sequence":"additional","affiliation":[{"name":"Periodontal Research Group and MRC Centre for Immune Regulation University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4898-040X","authenticated-orcid":false,"given":"Robert","family":"Insall","sequence":"additional","affiliation":[{"name":"Periodontal Research Group and MRC Centre for Immune Regulation University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Kalna","sequence":"additional","affiliation":[{"name":"Periodontal Research Group and MRC Centre for Immune Regulation University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Spengler","sequence":"additional","affiliation":[{"name":"Periodontal Research Group and MRC Centre for Immune Regulation University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa M.","family":"Grant","sequence":"additional","affiliation":[{"name":"Periodontal Research Group and MRC Centre for Immune Regulation University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain L.C.","family":"Chapple","sequence":"additional","affiliation":[{"name":"Periodontal Research Group and MRC Centre for Immune Regulation University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,1,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1989.tb00870.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1536"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.018077"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1155"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.45.3.249"},{"key":"e_1_2_6_7_1","first-page":"243","article-title":"Study of chemotactic activity developed by neutrophils from rheumatoid arthritis patients","volume":"61","author":"Bostan M.","year":"2002","journal-title":"Roumanian Archives of Microbiology and Immunology"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2004.00509.x"},{"key":"e_1_2_6_9_1","first-page":"2162","article-title":"Intracellular oxidative activation in synovial fluid neutrophils from patients with rheumatoid arthritis but not from other arthritis patients","volume":"34","author":"Cedergren J.","year":"2007","journal-title":"Journal of Rheumatology"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1998.69.2.209"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2006.01029.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12077"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/137.3.657"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.435313"},{"key":"e_1_2_6_15_1","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1128\/iai.18.3.694-700.1977","article-title":"Defective neutrophil chemotaxis in juvenile periodontitis","volume":"18","author":"Clark R. 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