{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T21:30:25Z","timestamp":1772227825880,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,2,16]],"date-time":"2015-02-16T00:00:00Z","timestamp":1424044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"ITI Foundation for the Promotion of Implantology, Basel, Switzerland"},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81271108"],"award-info":[{"award-number":["81271108"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The local delivery of growth factors via gene therapy has gained tremendous awareness in recent years due to their sustained growth factor delivery to target tissues. The aim of this study was to fabricate and investigate a scaffold able to release growth factors via gene therapy for the repair of periodontal tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Novel mesoporous bioglass (<jats:styled-content style=\"fixed-case\">MBG<\/jats:styled-content>)\/silk fibrin scaffold combined with <jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>7 and\/or <jats:styled-content style=\"fixed-case\">PDGF<\/jats:styled-content>\u2010B adenovirus was fabricated and tested in vitro for cell migration, proliferation and differentiation. Furthermore, acute\u2010type buccal dehiscence periodontal defects (mesiodistal width\u00a0\u00d7\u00a0depth: 5\u00a0\u00d7\u00a05\u00a0mm) were created on the buccal portion of the maxillary premolars in five normal male beagle dogs (12\u00a0months old, 15.0\u00a0\u00b1\u00a02.0\u00a0kg) and histologically examined for periodontal regeneration following implantation of the following five groups: (1) no scaffold, (2) <jats:styled-content style=\"fixed-case\">MBG<\/jats:styled-content>\/silk scaffold alone, (3) scaffold\u00a0+\u00a0ad<jats:styled-content style=\"fixed-case\">PDGF<\/jats:styled-content>\u2010B, (4) scaffold\u00a0+ ad<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>7, (5) scaffold\u00a0+\u00a0ad<jats:styled-content style=\"fixed-case\">PDGF<\/jats:styled-content>\u2010b\u00a0+\u00a0ad<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>7.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In vitro findings demonstrated that ad<jats:styled-content style=\"fixed-case\">PDGF<\/jats:styled-content>\u2010B was able to rapidly recruit periodontal ligament (<jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>) cells over sixfold more effectively than ad<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>7, whereas ad<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>7 was more able to induce osteoblast differentiation of <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> cells. In vivo findings demonstrate that scaffolds loaded with ad<jats:styled-content style=\"fixed-case\">PDGF<\/jats:styled-content>\u2010B were able to partially regenerate the periodontal ligament while ad<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>7 scaffolds primarily improved new bone formation. The combination of both ad<jats:styled-content style=\"fixed-case\">PDGF<\/jats:styled-content>\u2010B and ad<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>7 synergistically promoted periodontal regeneration by allowing up to two times greater regeneration of the periodontal ligament, alveolar bone and cementum when compared to each adenovirus used alone.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Although both <jats:styled-content style=\"fixed-case\">PDGF<\/jats:styled-content>\u2010B and <jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>7 are individually capable of promoting periodontal regeneration to some degree, their combination synergistically promotes wound healing in acute\u2010type buccal dehiscence periodontal defects when delivered simultaneously. This study demonstrates the promise for successful delivery of low\u2010cost, effective growth factor delivery via gene therapy for the treatment of periodontal defects.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12364","type":"journal-article","created":{"date-parts":[[2015,1,9]],"date-time":"2015-01-09T15:51:54Z","timestamp":1420818714000},"page":"262-271","source":"Crossref","is-referenced-by-count":42,"title":["Novel <scp>M<\/scp>eso<scp>P<\/scp>orous <scp>B<\/scp>ioGlass\/silk scaffold containing ad<scp>PDGF<\/scp>\u2010B and ad<scp>BMP<\/scp>7 for the repair of periodontal defects in beagle dogs"],"prefix":"10.1111","volume":"42","author":[{"given":"Yufeng","family":"Zhang","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Oral Implantology School of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard J.","family":"Miron","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Periodontology School of Dental Medicine University of Bern Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sue","family":"Li","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Shi","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Oral Implantology School of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anton","family":"Sculean","sequence":"additional","affiliation":[{"name":"Department of Periodontology School of Dental Medicine University of Bern Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangrong","family":"Cheng","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; 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