{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:42:40Z","timestamp":1790023360376,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,5,14]],"date-time":"2015-05-14T00:00:00Z","timestamp":1431561600000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Christian-Albrechts-University at Kiel, Germany"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2015,5]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>This study investigated the periodontal regenerative potential of gingival margin\u2010derived stem\/progenitor cells (G<\/jats:styled-content>\u2010MSC<\/jats:styled-content>s) in conjunction with IL<\/jats:styled-content>\u20101ra\u2010releasing hyaluronic acid synthetic extracellular matrix (HA<\/jats:styled-content>\u2010sECM<\/jats:styled-content>).<\/jats:p><\/jats:sec>Materials and Methods<\/jats:title>Periodontal defects were induced at four sites in eight miniature pigs in the premolar\/molar area (\u22124\u00a0weeks). Autologus G<\/jats:styled-content>\u2010MSC<\/jats:styled-content>s were isolated from the free gingival margin and magnetically sorted, using anti\u2010STRO<\/jats:styled-content>\u20101 antibodies. Colony formation and multilineage differentiation potential were tested. The G<\/jats:styled-content>\u2010MSC<\/jats:styled-content>s were expanded and incorporated into IL<\/jats:styled-content>\u20101ra\u2010loaded\/unloaded HA<\/jats:styled-content>\u2010sECM<\/jats:styled-content>. Within every miniature pig, four periodontal defects were randomly treated with IL<\/jats:styled-content>\u20101ra\/G<\/jats:styled-content>\u2010MSC<\/jats:styled-content>s\/HA<\/jats:styled-content>\u2010sECM<\/jats:styled-content> (test group), G<\/jats:styled-content>\u2010MSC<\/jats:styled-content>s\/HA<\/jats:styled-content>\u2010sECM<\/jats:styled-content> (positive\u2010control), scaling and root planing (SRP<\/jats:styled-content>; negative control\u20101) or left untreated (no\u2010treatment group; negative control 2). Differences in clinical attachment level (\u0394CAL<\/jats:styled-content>), probing depth (\u0394PD<\/jats:styled-content>), gingival recession (\u0394GR<\/jats:styled-content>), radiographic defect volume (\u0394RDV<\/jats:styled-content>), and changes in bleeding on probing (BOP<\/jats:styled-content>) between baseline and 16 weeks post\u2010transplantation, as well as periodontal attachment level (PAL<\/jats:styled-content>), junctional epithelium length (JE<\/jats:styled-content>), connective tissue adhesion (CTA<\/jats:styled-content>), cementum regeneration (CR<\/jats:styled-content>) and bone regeneration (BR<\/jats:styled-content>) at 16 weeks post\u2010transplantation were evaluated.<\/jats:p><\/jats:sec>Results<\/jats:title>Isolated G<\/jats:styled-content>\u2010MSC<\/jats:styled-content>s showed stem\/progenitor cell characteristics. IL<\/jats:styled-content>\u20101ra loaded and unloaded G<\/jats:styled-content>\u2010MSC<\/jats:styled-content>s\/HA<\/jats:styled-content>\u2010sECM<\/jats:styled-content> showed higher \u0394CAL<\/jats:styled-content>, \u0394PD<\/jats:styled-content>, \u0394GR<\/jats:styled-content>, PAL<\/jats:styled-content>, CR<\/jats:styled-content> and BR<\/jats:styled-content> as well as a lower JE<\/jats:styled-content> compared to their negative controls and improved BOP<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>G<\/jats:styled-content>\u2010MSC<\/jats:styled-content>s in conjunction with IL<\/jats:styled-content>\u20101ra\u2010loaded\/unloaded HA<\/jats:styled-content>\u2010sECM<\/jats:styled-content> show a significant periodontal regenerative potential.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12401","type":"journal-article","created":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T12:29:07Z","timestamp":1429100947000},"page":"448-457","source":"Crossref","is-referenced-by-count":90,"title":["Periodontal regeneration employing gingival margin\u2010derived stem\/progenitor cells in conjunction with IL<\/scp>\u20101ra\u2010hydrogel synthetic extracellular matrix"],"prefix":"10.1111","volume":"42","author":[{"given":"Karim M.","family":"Fawzy El\u2010Sayed","sequence":"first","affiliation":[{"name":"Clinic for Conservative Dentistry and Periodontology School of Dental Medicine Christian Albrechts University Kiel Germany"},{"name":"Oral Medicine and Periodontology Department Faculty of Oral and Dental Medicine Cairo University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed K.","family":"Mekhemar","sequence":"additional","affiliation":[{"name":"Clinic for Conservative Dentistry and Periodontology School of Dental Medicine Christian Albrechts University Kiel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedicta E.","family":"Beck\u2010Broichsitter","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Telse","family":"B\u00e4hr","sequence":"additional","affiliation":[{"name":"Animal Experiment Division Clinic for Heart\u2010 and Vascular\u2010Surgery University Hospital Schleswig\u2010Holstein Kiel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa","family":"Hegab","sequence":"additional","affiliation":[{"name":"Clinic for Conservative Dentistry and Periodontology School of Dental Medicine Christian Albrechts University Kiel 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