{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:27:58Z","timestamp":1791268078669,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,11,29]],"date-time":"2015-11-29T00:00:00Z","timestamp":1448755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI11\/01383"],"award-info":[{"award-number":["PI11\/01383"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To analyse the relationship between the chronic periodontitis\u2010associated subgingival microbiota and clinical inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Sixty subjects with generalized chronic periodontitis participated in this study. Patients were divided into two groups according to their bleeding on probing (<jats:styled-content style=\"fixed-case\">BOP<\/jats:styled-content>) scores: <jats:styled-content style=\"fixed-case\">BOP<\/jats:styled-content>\u20101 group (mean scores \u226450% in sampled sites) and <jats:styled-content style=\"fixed-case\">BOP<\/jats:styled-content>\u20102 group (mean scores &gt;50%). Subgingival bacterial samples from periodontal patients were studied by pyrosequencing <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> products of the 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene and by real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In all the analysed subgingival samples, 102 bacterial genera and 203 species (from 41 genera of interest) were identified. Rarefaction curves showed a greater number of bacterial species in samples from <jats:styled-content style=\"fixed-case\">BOP<\/jats:styled-content>\u20102 group compared to <jats:styled-content style=\"fixed-case\">BOP<\/jats:styled-content>\u20101 group. The <jats:styled-content style=\"fixed-case\">BOP<\/jats:styled-content>\u20101 group had significantly higher abundance percentages of <jats:italic>Anaeroglobus<\/jats:italic> (especifically, <jats:italic>A.\u00a0geminatus<\/jats:italic>), <jats:italic>Capnocytophaga<\/jats:italic> (especifically <jats:italic>C.\u00a0gingivalis<\/jats:italic>), <jats:italic><jats:styled-content style=\"fixed-case\">TM<\/jats:styled-content>7<\/jats:italic> and <jats:italic>Veillonella<\/jats:italic>. The <jats:styled-content style=\"fixed-case\">BOP<\/jats:styled-content>\u20102 had significantly higher abundance percentages of <jats:italic>Desulfobulbus<\/jats:italic> (especially <jats:italic>D.\u00a0propionicus), Eubacterium<\/jats:italic> (especially <jats:italic>E.\u00a0saphenum), Filifactor alocis, Streptococcus constellatus, Tannerella<\/jats:italic> (especially<jats:italic>, T.\u00a0forsythia<\/jats:italic>) and <jats:italic>Treponema<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>16S pyrosequencing revealed that increased inflammation, at sites with periodontitis, is associated with a more diverse subgingival microbiota and specific changes in the bacterial composition, involving \u201cestablished\u201d periopathogens, symbionts and novel low\u2010abundance pathobionts.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12470","type":"journal-article","created":{"date-parts":[[2015,10,13]],"date-time":"2015-10-13T17:36:52Z","timestamp":1444757812000},"page":"1074-1082","source":"Crossref","is-referenced-by-count":83,"title":["Relationship between periodontitis\u2010associated subgingival microbiota and clinical inflammation by 16S pyrosequencing"],"prefix":"10.1111","volume":"42","author":[{"given":"A","family":"Camelo\u2010Castillo","sequence":"first","affiliation":[{"name":"FISABIO Foundation Centre for Advanced Research in Public Health Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Novoa","sequence":"additional","affiliation":[{"name":"Special Needs Unit and Periodontology Unit Oral Sciences Research Group School of Medicine and Dentistry University of Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Balsa\u2010Castro","sequence":"additional","affiliation":[{"name":"Special Needs Unit and Periodontology Unit Oral Sciences Research Group School of Medicine and Dentistry University of Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Blanco","sequence":"additional","affiliation":[{"name":"Special Needs Unit and Periodontology Unit Oral Sciences Research Group School of Medicine and Dentistry University of Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Mira","sequence":"additional","affiliation":[{"name":"FISABIO Foundation Centre for Advanced Research in Public Health Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Tom\u00e1s","sequence":"additional","affiliation":[{"name":"Special Needs Unit and Periodontology Unit Oral Sciences Research Group School of Medicine and Dentistry University of Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,11,29]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.174"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/adj.12076"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12210"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.85"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057782"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0512-6_2"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.3.1687-1694.2003"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00119"},{"key":"e_1_2_5_13_1","first-page":"baq013","article-title":"The Human Oral Microbiome Database: a web accessible resource for investigating oral microbe taxonomic and genomic information","volume":"6","author":"Chen T.","year":"2010","journal-title":"Database (Oxford)"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn879"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2014.08.017"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2011.00397.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065520"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.191"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2012.00663.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201344202"},{"key":"e_1_2_5_21_1","unstructured":"Holland S. 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