{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:41:41Z","timestamp":1791416501468,"version":"4.3.3"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,12,21]],"date-time":"2015-12-21T00:00:00Z","timestamp":1450656000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001718","name":"Netherlands Organization for International Cooperation in Higher Education","doi-asserted-by":"publisher","award":["CF 7454"],"award-info":[{"award-number":["CF 7454"]}],"id":[{"id":"10.13039\/501100001718","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001827","name":"Universiteit van Amsterdam","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001827","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess in a periodontally diseased rural population deprived from regular dental care and having poor dietary conditions, the effect of vitamin C\/calcium threonate\/citrus flavonoids (VitC\/Ca\/Fl) supplementation on subgingival microbiota and plasma levels of vitamin C, HbA1c and hs<jats:styled-content style=\"fixed-case\">CRP<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material &amp; Methods<\/jats:title><jats:p>The <jats:bold>s<\/jats:bold>tudy population consisted of 98 subjects who previously participated in a prospective study on the natural history of periodontitis. Participants were instructed to consume one tablet\/day containing 200\u00a0mg Ester C<jats:sup>\u00ae<\/jats:sup> calcium ascorbate, 25\u00a0mg calcium threonate and 100\u00a0mg citrus flavonoids for 90\u00a0days. Following parameters were evaluated: prevalence\/amount of seven traditional periodontal pathogens, cytomegalovirus, Epstein\u2013Barr virus (<jats:styled-content style=\"fixed-case\">EBV<\/jats:styled-content>); and plasma levels of vitamin C, HbA1c and hs<jats:styled-content style=\"fixed-case\">CRP<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After VitC\/Ca\/Fl supplementation, 100% of subjects showed normal plasma vitamin C values compared to 55% before. At baseline, 48% of subjects harboured <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic>, &gt;97% the other periodontal pathogens and 73% <jats:styled-content style=\"fixed-case\">EBV<\/jats:styled-content>. Supplementation with VitC\/Ca\/F reduced the subgingival load of all studied bacteria (<jats:italic>p<\/jats:italic>\u2010values: 0.014\u20130.0001) and <jats:styled-content style=\"fixed-case\">EBV<\/jats:styled-content> (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.0001) substantially in all initially positive subjects. Plasma levels of HbA1c and hs<jats:styled-content style=\"fixed-case\">CRP<\/jats:styled-content> dropped in all subjects (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This uncontrolled study suggested that supplemental VitC\/Ca\/Fl may be helpful in reducing subgingival numbers of periodontal pathogens and <jats:styled-content style=\"fixed-case\">EBV<\/jats:styled-content>, and promoting systemic health.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12478","type":"journal-article","created":{"date-parts":[[2015,11,9]],"date-time":"2015-11-09T10:42:46Z","timestamp":1447065766000},"page":"1097-1104","source":"Crossref","is-referenced-by-count":11,"title":["Java project on periodontal diseases: effect of vitamin <scp>C<\/scp>\/calcium threonate\/citrus flavonoids supplementation on periodontal pathogens, <scp>CRP<\/scp> and <scp>H<\/scp>b<scp>A<\/scp>1c"],"prefix":"10.1111","volume":"42","author":[{"given":"Amaliya","family":"Amaliya","sequence":"first","affiliation":[{"name":"Department of Periodontology Padjadjaran State University Bandung Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marja L.","family":"Laine","sequence":"additional","affiliation":[{"name":"Department of Periodontology Academic Centre for Dentistry Amsterdam (ACTA) University of Amsterdam and VU University Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno G.","family":"Loos","sequence":"additional","affiliation":[{"name":"Department of Periodontology Academic Centre for Dentistry Amsterdam (ACTA) University of Amsterdam and VU University Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ubele","family":"Van der Velden","sequence":"additional","affiliation":[{"name":"Department of Periodontology Academic Centre for Dentistry Amsterdam (ACTA) University of Amsterdam and VU University Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2007.01053.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12381"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2004.00643.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc12-s064"},{"key":"e_1_2_6_6_1","first-page":"637","article-title":"The effect of quercetin supplementation on selected markers of inflammation and oxidative stress","volume":"17","author":"Askari G.","year":"2012","journal-title":"Journal of Research in Medical Sciences"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf8006568"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12087"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu7042574"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000314597"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/137.3.657"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041594"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.41.2.167"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12253"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(91)90388-R"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.112.173187"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/19390211.2013.790332"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02850358"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3785"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.2105\/AJPH.94.5.870"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.168.14.1493"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1987.tb23772.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.2011.10719993"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2010.513779"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345910700121101"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1986.57.8.480"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.8.3704"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.111.145334"},{"issue":"1","key":"e_1_2_6_29_1","first-page":"S361","article-title":"Effects of eicosapentaenoic acid and vitamin C on glycemic indices, blood pressure, and serum lipids in type 2 diabetic Iranian males","volume":"16","author":"Mahmoudabadi M. M.","year":"2011","journal-title":"Journal of Research in Medical Sciences"},{"key":"e_1_2_6_30_1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1093\/oxfordjournals.epirev.a036022","article-title":"Epidemiology of acute necrotizing ulcerative gingivitis","volume":"10","author":"Melnick S. L.","year":"1988","journal-title":"Epidemiological Reviews"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncpcardio1322"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000052939.59093.45"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms140817221"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bdj.4812613"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2458-8-39"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2001.028007617.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11883-013-0368-y"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2006.00953.x"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(91)90343-A"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn.83.3.567"},{"key":"e_1_2_6_41_1","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1093\/ajcn\/70.1.49","article-title":"Serum vitamin C concentrations and diabetes: findings from the Third National Health and Nutrition Examination Survey, 1988\u20131994","volume":"70","author":"Will J. C.","year":"1999","journal-title":"American Journal of Clinical Nutrition"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc13-0877"}],"container-title":["Journal of Clinical Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcpe.12478","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcpe.12478","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.12478","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T08:31:35Z","timestamp":1696149095000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcpe.12478"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12]]},"references-count":41,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1111\/jcpe.12478"],"URL":"https:\/\/doi.org\/10.1111\/jcpe.12478","archive":["Portico"],"relation":{},"ISSN":["0303-6979","1600-051X"],"issn-type":[{"value":"0303-6979","type":"print"},{"value":"1600-051X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12]]}}}