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Matrix metalloproteinases (MMP<\/jats:styled-content>s) \u20102 and \u20109 were determined by zymography; levels of MMP<\/jats:styled-content>\u20108 by ELISA<\/jats:styled-content> and IFMA<\/jats:styled-content> and MPO<\/jats:styled-content> by ELISA<\/jats:styled-content>. IL<\/jats:styled-content>\u20101, IL<\/jats:styled-content>\u20106, TNF<\/jats:styled-content>\u03b1<\/jats:italic>, DKK<\/jats:styled-content>\u20101, Osteonectin, Periostin, TRAP<\/jats:styled-content>\u20105 and OPG<\/jats:styled-content> were determined by a multiplex quantitative panel. Statistical analysis was performed using linear mixed\u2010effects models.<\/jats:p><\/jats:sec>Results<\/jats:title>The MMP<\/jats:styled-content>\u20109 and MMP<\/jats:styled-content>\u20108 were higher in CP<\/jats:styled-content>, followed by AAP<\/jats:styled-content>, versus<\/jats:italic> healthy individuals (p<\/jats:italic>\u00a0<\u00a00.05). ProMMP<\/jats:styled-content>\u20102, MPO<\/jats:styled-content>, IL<\/jats:styled-content>\u20101, IL<\/jats:styled-content>\u20106, PTN<\/jats:styled-content>, TRAP<\/jats:styled-content>\u20105 and OPG<\/jats:styled-content> were significantly higher in CP<\/jats:styled-content> when compared with AAP<\/jats:styled-content> and healthy patients (p<\/jats:italic>\u00a0<\u00a00.05). The highest diagnostic accuracies were observed for ProMMP<\/jats:styled-content>\u20102, ProMMP<\/jats:styled-content>\u20109, MMP<\/jats:styled-content>\u20108 and TRAP<\/jats:styled-content>\u20105 (AUC<\/jats:styled-content>\u00a0>\u00a00.97) in CP<\/jats:styled-content>, and for the active form of MMP<\/jats:styled-content>\u20109 and MMP<\/jats:styled-content>\u20108 (AUC<\/jats:styled-content>\u00a0>\u00a00.90) in AAP<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Gingival crevicular fluid composition is modified by CP<\/jats:styled-content> and AAP<\/jats:styled-content>. MMP<\/jats:styled-content>\u20109 and MMP<\/jats:styled-content>\u20108 show diagnostic potential for CP<\/jats:styled-content> and AAP<\/jats:styled-content>, whereas MMP<\/jats:styled-content>\u20102 and TRAP<\/jats:styled-content>\u20105 are useful only for CP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12479","type":"journal-article","created":{"date-parts":[[2015,11,11]],"date-time":"2015-11-11T11:51:20Z","timestamp":1447242680000},"page":"34-45","source":"Crossref","is-referenced-by-count":86,"title":["Diagnostic accuracy for apical and chronic periodontitis biomarkers in gingival crevicular fluid: an exploratory study"],"prefix":"10.1111","volume":"43","author":[{"given":"Mauricio","family":"Baeza","sequence":"first","affiliation":[{"name":"Laboratory of Periodontal Biology Department of Conservative Dentistry Faculty of Dentistry University of Chile Santiago Chile"},{"name":"Department of Conservative Dentistry Faculty of Dentistry University of Chile 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