{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:24:17Z","timestamp":1790839457917,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,3,2]],"date-time":"2016-03-02T00:00:00Z","timestamp":1456876800000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"KAKENHI","award":["26861685"],"award-info":[{"award-number":["26861685"]}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for Promotion of Science","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of this study was to investigate the effects of a self\u2010assembling peptide (<jats:styled-content style=\"fixed-case\">SAP<\/jats:styled-content>) nanofibre hydrogel on healing of surgical periodontal defects in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>In vitro interactions between rat periodontal ligament (<jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>) cells and <jats:styled-content style=\"fixed-case\">SAP<\/jats:styled-content> hydrogel (2.5% <jats:styled-content style=\"fixed-case\">RADA<\/jats:styled-content>16) were assessed by cell proliferation assays. In vivo, maxillary first molars of 45 Wistar rats were extracted and after healing, bilateral periodontal defects were surgically created mesially in second molars. Defects were treated with <jats:styled-content style=\"fixed-case\">RADA<\/jats:styled-content>16, Matrigel, or left unfilled. After 2 and 4\u00a0weeks, defect healing was evaluated by microcomputed tomography, histological and immunohistochemical analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Periodontal ligament cells grown on <jats:styled-content style=\"fixed-case\">RADA<\/jats:styled-content>16 showed an gradual increase in proliferation up to 72\u00a0h. At 4\u00a0weeks post surgery, the bone volume fraction and trabecular thickness of defect areas in the <jats:styled-content style=\"fixed-case\">RADA<\/jats:styled-content>16 group were significantly greater than those in other groups. Histologically, enhanced new bone formation was observed in the <jats:styled-content style=\"fixed-case\">RADA<\/jats:styled-content>16 group. At 4\u00a0weeks, <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>\u2010like collagen bundles ran oblique to the root surface in the <jats:styled-content style=\"fixed-case\">RADA<\/jats:styled-content>16 group. Expression levels of <jats:styled-content style=\"fixed-case\">PCNA<\/jats:styled-content>\u2010positive cells, vascular endothelial growth factor and osteopontin in the <jats:styled-content style=\"fixed-case\">RADA<\/jats:styled-content>16 group were significantly greater than those in other groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Within the limitations of the study, application of the <jats:styled-content style=\"fixed-case\">SAP<\/jats:styled-content> hydrogel promoted healing of surgical periodontal defects by enhancing cell recruitment and possibly angiogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12515","type":"journal-article","created":{"date-parts":[[2016,1,20]],"date-time":"2016-01-20T16:00:32Z","timestamp":1453305632000},"page":"279-288","source":"Crossref","is-referenced-by-count":34,"title":["Enhanced healing of surgical periodontal defects in rats following application of a self\u2010assembling peptide nanofibre 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