{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:01:19Z","timestamp":1789513279064,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,4,6]],"date-time":"2016-04-06T00:00:00Z","timestamp":1459900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["#8P20GM103538\u201009"],"award-info":[{"award-number":["#8P20GM103538\u201009"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Cellular and molecular immunoinflammatory changes in gingival tissues drive alveolar bone loss in periodontitis. Since ageing is a risk factor for periodontitis, we sought to identify age\u2010related gingival transcriptome changes associated with bone metabolism in both healthy and in naturally occurring periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Adult (12\u201316\u00a0years) and aged (18\u201323\u00a0years) non\u2010human primates (<jats:italic>M. mulatta<\/jats:italic>) (<jats:italic>n<\/jats:italic>\u00a0=\u00a024) were grouped into healthy and periodontitis. Gingival tissue samples were obtained and subjected to microarray analysis using the Gene Chip Macaque Genome Array. Gene expression profiles involved in osteoclast\/osteoblast proliferation, adhesion and function were evaluated and compared across and between the age groups. <jats:styled-content style=\"fixed-case\">QPCR<\/jats:styled-content> was also performed on selected genes to validate microarray data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Healthy aged tissues showed a gene profile expression that suggest enhancement of osteoclastic adhesion, proliferation\/survival and function (<jats:styled-content style=\"fixed-case\">SPP<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>4, <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>8 and <jats:styled-content style=\"fixed-case\">TFEC<\/jats:styled-content>) and impaired osteoblastic activity (<jats:styled-content style=\"fixed-case\">SMEK<\/jats:styled-content>3P and <jats:styled-content style=\"fixed-case\">SMAD<\/jats:styled-content>5). The gingival transcriptome in both adult and aged animals with naturally occurring periodontitis (<jats:styled-content style=\"fixed-case\">FOS<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> IL<\/jats:styled-content>6, <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>4, <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>9, <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>10 and <jats:styled-content style=\"fixed-case\">SPP<\/jats:styled-content>1 genes) was consistent with a local inflammatory response driving towards bone\/connective tissue destruction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A pro\u2010osteoclastogenic gingival transcriptome is associated with periodontitis irrespective of age; however; a greater bone\u2010destructive molecular environment is associated with ageing in healthy tissues.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12528","type":"journal-article","created":{"date-parts":[[2016,2,9]],"date-time":"2016-02-09T16:36:55Z","timestamp":1455035815000},"page":"408-417","source":"Crossref","is-referenced-by-count":31,"title":["Bone biology\u2010related gingival transcriptome in ageing and periodontitis in non\u2010human primates"],"prefix":"10.1111","volume":"43","author":[{"given":"Subramanya N.","family":"Pandruvada","sequence":"first","affiliation":[{"name":"Division of Orthodontics College of Dentistry University of Kentucky Lexington KY USA"},{"name":"Center for Oral Health Research College of Dentistry University of Kentucky Lexington KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Octavio A.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Center for Oral Health Research College of Dentistry University of Kentucky Lexington KY 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