{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T04:28:04Z","timestamp":1776918484947,"version":"3.51.2"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,5,4]],"date-time":"2016-05-04T00:00:00Z","timestamp":1462320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To determine whether haemolytic activity of subgingival bacteria is associated with periodontitis clinical parameters and to identify which bacteria produce the haemolysins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Subgingival plaque samples from 22 untreated chronic periodontitis patients were investigated by culture and identified with matrix assisted laser desorption\/ionisation time\u2010of\u2010flight mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Total aerobic and anaerobic bacterial viable counts, percentage distribution of <jats:italic>\u03b1<\/jats:italic>\u2010 and <jats:italic>\u03b2<\/jats:italic>\u2010haemolytic bacteria were significantly elevated in diseased sites in relation to healthy sites (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001). Periodontal pathogens were more frequently detected at diseased sites: <jats:italic>Porphyromonas gingivalis, Tannerella forsythia, Treponema<\/jats:italic> sp., <jats:italic>Prevotella<\/jats:italic> sp., <jats:italic>Parvimonas micra<\/jats:italic>,<jats:italic> Fusobacterium<\/jats:italic> sp., <jats:italic>Campylobacter<\/jats:italic> sp., <jats:italic>Capnocytophaga<\/jats:italic> sp., and <jats:italic>Selenomonas<\/jats:italic> sp. Haemolytic unidentifiable species and Gram\u2010positive anaerobes such as <jats:italic>Slackia exigua<\/jats:italic>,<jats:italic> Solobacterium moorei<\/jats:italic>, and <jats:italic>Bulledia extructa<\/jats:italic> were also more frequently detected at diseased sites. In diseased sites, the presence of different haemolytic characteristics was more strongly correlated with clinical measures of disease than the mere absence or presence of specific species. The strongest correlation with probing pocket depth was observed for overall <jats:italic>\u03b2<\/jats:italic>\u2010haemolytic toxicity (<jats:italic>r\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.73, <jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A strong association was observed between subgingival bacterial haemolytic activity and clinical parameters in patients with chronic periodontitis. Further investigations are warranted to delineate the role of haemolysins in the pathogenesis of periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12554","type":"journal-article","created":{"date-parts":[[2016,4,23]],"date-time":"2016-04-23T08:53:47Z","timestamp":1461401627000},"page":"503-511","source":"Crossref","is-referenced-by-count":13,"title":["Association of clinical parameters with periodontal bacterial haemolytic activity"],"prefix":"10.1111","volume":"43","author":[{"given":"Benedicta K. J.","family":"Wong","sequence":"first","affiliation":[{"name":"Melbourne Dental School Faculty of Medicine Dentistry and Health Sciences The University of Melbourne Carlton Vic. Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil R.","family":"McGregor","sequence":"additional","affiliation":[{"name":"Melbourne Dental School Faculty of Medicine Dentistry and Health Sciences The University of Melbourne Carlton Vic. Australia"},{"name":"Bioscreen (Aust) Pty Ltd Yarraville Vic. Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry L.","family":"Butt","sequence":"additional","affiliation":[{"name":"Bioscreen (Aust) Pty Ltd Yarraville Vic. Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel","family":"Knight","sequence":"additional","affiliation":[{"name":"Bioscreen (Aust) Pty Ltd Yarraville Vic. Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise Ye","family":"Liu","sequence":"additional","affiliation":[{"name":"Melbourne Dental School Faculty of Medicine Dentistry and Health Sciences The University of Melbourne Carlton Vic. Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan B.","family":"Darby","sequence":"additional","affiliation":[{"name":"Melbourne Dental School Faculty of Medicine Dentistry and Health Sciences The University of Melbourne Carlton Vic. 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