{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:04:49Z","timestamp":1790841889101,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,6,7]],"date-time":"2016-06-07T00:00:00Z","timestamp":1465257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000855","name":"University of Birmingham","doi-asserted-by":"publisher","award":["RG_10\u2010077"],"award-info":[{"award-number":["RG_10\u2010077"]}],"id":[{"id":"10.13039\/501100000855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      To determine peripheral blood neutrophil superoxide release and C\u2010reactive protein (\n                      <jats:styled-content style=\"fixed-case\">CRP<\/jats:styled-content>\n                      ) concentration in chronic periodontitis patients, before and after non\u2010surgical periodontal treatment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Neutrophils were isolated from patient and control volunteers (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a020) and superoxide measured by lucigenin\u2010enhanced chemiluminescence with and without stimulation with unopsonized\n                      <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                      , unopsonized\n                      <jats:italic>Fusobacterium nucleatum<\/jats:italic>\n                      and phorbol 12\u2010myristate 13\u2010acetate (\n                      <jats:styled-content style=\"fixed-case\">PMA<\/jats:styled-content>\n                      ) before and 2\u2010months following non\u2010surgical therapy. Corresponding high\u2010sensitivity plasma\n                      <jats:styled-content style=\"fixed-case\">CRP<\/jats:styled-content>\n                      concentrations were also determined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      At pre\u2010treatment baseline, patient neutrophils released more superoxide in the absence (\n                      <jats:italic>p\u00a0<\/jats:italic>\n                      \u2264\u00a00.032) and presence of periodontal bacteria (\n                      <jats:italic>p\u00a0<\/jats:italic>\n                      \u2264\u00a00.013) and after\n                      <jats:styled-content style=\"fixed-case\">PMA<\/jats:styled-content>\n                      stimulation (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.041) compared to control cells. Post\u2010therapy, patient neutrophil superoxide release was reduced to control cell levels. Median patient plasma\n                      <jats:styled-content style=\"fixed-case\">CRP<\/jats:styled-content>\n                      concentrations were non\u2010significantly higher than control values and were reduced after therapy (1.80\u20131.36\u00a0mg\/l). Patient pre\u2010treatment baseline, unstimulated neutrophil superoxide release showed a significant, positive correlation with plasma\n                      <jats:styled-content style=\"fixed-case\">CRP<\/jats:styled-content>\n                      concentration (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Chronic periodontitis is characterized by peripheral neutrophils exhibiting superoxide hyperactivity and hyper\u2010reactivity to periodontal pathogens that is not a constitutive feature of periodontitis patients. The positive, pre\u2010therapy relationship between unstimulated neutrophil superoxide release and plasma\n                      <jats:styled-content style=\"fixed-case\">CRP<\/jats:styled-content>\n                      is consistent with a protective role for\n                      <jats:styled-content style=\"fixed-case\">CRP<\/jats:styled-content>\n                      in reducing oxidative stress and systemic inflammation\n                      <jats:italic>in\u00a0vivo<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.12575","type":"journal-article","created":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T08:44:02Z","timestamp":1462956242000},"page":"652-658","source":"Crossref","is-referenced-by-count":49,"title":["Neutrophil superoxide release and plasma C\u2010reactive protein levels pre\u2010 and post\u2010periodontal therapy"],"prefix":"10.1111","volume":"43","author":[{"given":"Martin R.","family":"Ling","sequence":"first","affiliation":[{"name":"Periodontal Research Group and MRC Centre for Immune Regulation College of Medical and Dental Sciences The University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain L. 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