{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T18:23:31Z","timestamp":1790447011538,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T00:00:00Z","timestamp":1477526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/J500434\/1"],"award-info":[{"award-number":["MR\/J500434\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000297","name":"Royal College of Surgeons of England","doi-asserted-by":"publisher","award":["16209"],"award-info":[{"award-number":["16209"]}],"id":[{"id":"10.13039\/501100000297","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate <jats:italic>ex\u00a0vivo<\/jats:italic> peripheral neutrophil extracellular trap (<jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content>) production and their subsequent degradation by plasma in chronic periodontitis patients, and periodontally and systemically healthy\u2010matched controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Chronic periodontitis patient and control (<jats:italic>n<\/jats:italic>\u00a0=\u00a040 pairs) peripheral blood neutrophils (PBN) were stimulated for <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content> quantification. A subset of patients received non\u2010surgical periodontal therapy (<jats:italic>n<\/jats:italic>\u00a0=\u00a019) and <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content>s were quantified 3 months later alongside controls. Blood plasma was collected from patients and controls to quantify plasma\u2010induced <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content> degradation (<jats:italic>n<\/jats:italic>\u00a0=\u00a019 pairs). Subsequent experiments quantified plasma concentrations of <jats:styled-content style=\"fixed-case\">DN<\/jats:styled-content>ase\u20101, immunoglobulin G (IgG), free light chains (<jats:styled-content style=\"fixed-case\">FLC<\/jats:styled-content>s), and cystatin C.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No differences were observed in <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content> production between patients and controls. However, <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content> production decreased significantly in patients post\u2010treatment. Plasma <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content> degradation was significantly lower in patients than controls, which may be due to significantly reduced <jats:styled-content style=\"fixed-case\">DN<\/jats:styled-content>ase\u20101 levels as demonstrated, or potentially due to elevated IgG\/<jats:styled-content style=\"fixed-case\">FLC<\/jats:styled-content> concentrations in patients. <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content> degradation post\u2010periodontal treatment was comparable between patients and controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content> production was comparable between patients and controls; however, non\u2010surgical therapy causes attenuated <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content> degradation by plasma is impaired in untreated chronic periodontitis, potentially increasing the chronic <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content> burden, which may enhance antimicrobial function, or conversely, increase the risk of autoimmune\/inflammatory responses.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12628","type":"journal-article","created":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T08:38:56Z","timestamp":1475051936000},"page":"1041-1049","source":"Crossref","is-referenced-by-count":53,"title":["Peripheral blood neutrophil extracellular trap production and degradation in chronic periodontitis"],"prefix":"10.1111","volume":"43","author":[{"given":"Phillipa","family":"White","sequence":"first","affiliation":[{"name":"Periodontal Research Group and MRC Centre for Immune Regulation School of Dentistry, University of Birmingham, UK and Birmingham Community Healthcare Trust 5 Mill Pool Way Birmingham B5 7EG 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