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The involvement of 5\u2010LO<\/jats:styled-content> metabolites in periodontal disease (PD<\/jats:styled-content>) is not well defined. This study aimed to assess the role of 5\u2010LO<\/jats:styled-content> in experimental PD<\/jats:styled-content> induced by Aggregatibacter actinomycetemcomitans<\/jats:italic> (Aa<\/jats:italic>).<\/jats:p><\/jats:sec>Material and Methods<\/jats:title>In vivo experiments were carried out using SV<\/jats:styled-content>129 wild\u2010type (WT<\/jats:styled-content>) and 5\u2010LO<\/jats:styled-content>\u2010deficient (5lo<\/jats:italic>\u2010\/\u2010<\/jats:sup>) mice inoculated with Aa<\/jats:italic>. Osteoclasts were stimulated in vitro with Aa<\/jats:italic>LPS<\/jats:styled-content> in the presence or not of selective inhibitors of the 5\u2010LO<\/jats:styled-content> pathway, or LTB<\/jats:styled-content>4 or platelet\u2010activating factor (PAF<\/jats:styled-content>), as PAF<\/jats:styled-content> has already been shown to increase osteoclast activity.<\/jats:p><\/jats:sec>Results<\/jats:title>In 5lo<\/jats:italic>\u2010\/\u2010<\/jats:italic><\/jats:sup> mice, there were no loss of alveolar bone and less TRAP<\/jats:styled-content>\u2010positive osteoclasts in periodontal tissues, after Aa<\/jats:italic> inoculation, despite local production of TNF<\/jats:styled-content>\u2010\u03b1 and IL<\/jats:styled-content>\u20106. The differentiation and activity of osteoclasts stimulated with Aa<\/jats:italic>LPS<\/jats:styled-content> were diminished in the presence of BLT<\/jats:styled-content>1 antagonist or 5\u2010LO<\/jats:styled-content> inhibitor, but not in the presence of cysteinyl leukotriene receptor antagonist. The osteoclast differentiation induced by PAF<\/jats:styled-content> was impaired by the BLT<\/jats:styled-content>1 antagonism.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>In conclusion, LTB<\/jats:styled-content>4 but not CysLT<\/jats:styled-content>s is important for Aa\u2010<\/jats:italic>induced alveolar bone loss. Overall, LTB<\/jats:styled-content>4 affects osteoclast differentiation and activity and is a key intermediate of PAF<\/jats:styled-content>\u2010induced osteoclastogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12755","type":"journal-article","created":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T13:38:49Z","timestamp":1496324329000},"page":"793-802","source":"Crossref","is-referenced-by-count":7,"title":["The role of 5\u2010lipoxygenase in Aggregatibacter actinomycetemcomitans<\/i>\u2010induced alveolar bone loss"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1770-9978","authenticated-orcid":false,"given":"Mila F. 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