{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:09:43Z","timestamp":1787015383898,"version":"build-2736575974"},"reference-count":52,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T00:00:00Z","timestamp":1502928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Italian Society of Periodontology Meeting"},{"name":"Goldman Research Prize"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To evaluate the influence of periodontal therapy on\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      methylation in patients with chronic periodontitis as compared to healthy individuals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>\n                      Twenty patients were enrolled into two groups: (i) 10 diagnosed as clinically healthy; and (ii) 10 diagnosed with chronic periodontitis. Clinical measures were recorded and gingival biopsies were harvested at baseline (both patient groups) and at 2 and 8\u00a0weeks post\u2010baseline for diseased individuals. Molecular\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      methylation analysis was performed by pyrosequencing for the putative inflammation\u2010associated genes\n                      <jats:styled-content style=\"fixed-case\">LINE<\/jats:styled-content>\n                      \u20101,\n                      <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\n                      \u20102,\n                      <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\n                      \u2010\u03b3 and\n                      <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\n                      \u2010\u03b1. Random\u2010intercept linear regression models were applied to evaluate methylation levels across groups at baseline and the methylation changes over time in the diseased and normal tissues.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Periodontal therapy did not influence gene expression methylation of\n                      <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\n                      \u2010\u03b1,\n                      <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\n                      \u2010\u03b3 and\n                      <jats:styled-content style=\"fixed-case\">LINE<\/jats:styled-content>\n                      \u20101 levels at normal and periodontitis sites over time. However, it significantly reduced\n                      <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\n                      \u20102 methylation levels comparable to healthy individuals at both 2 and 8\u00a0weeks post\u2010treatment (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Periodontal therapy resets the\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      methylation status of inflammatory gene for\n                      <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\n                      \u20102 in patients with periodontal disease.\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      methylation levels of\n                      <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\n                      \u2010\u03b1,\n                      <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\n                      \u2010\u03b3 and\n                      <jats:styled-content style=\"fixed-case\">LINE<\/jats:styled-content>\n                      \u20101 were sustained in periodontitis sites despite therapy. Future studies should consider an expanded panel of inflammatory genes over time. (ClinicalTrials.gov\n                      <jats:styled-content style=\"fixed-case\">NCT<\/jats:styled-content>\n                      02835898).\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.12783","type":"journal-article","created":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T03:42:38Z","timestamp":1500867758000},"page":"905-914","source":"Crossref","is-referenced-by-count":35,"title":["Evaluation of\n                    <scp>DNA<\/scp>\n                    methylation of inflammatory genes following treatment of chronic periodontitis: A pilot case\u2013control study"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5186-2645","authenticated-orcid":false,"given":"Farah","family":"Asa'ad","sequence":"first","affiliation":[{"name":"Department of Biomedical, Surgical and Dental Sciences Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico University of Milan  Milan 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of Epithelial Biology University of Michigan School of Dentistry  Ann Arbor MI USA"},{"name":"Department of Periodontics and Oral Medicine School of Dentistry University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Rossi","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Surgical and Dental Sciences Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico University of Milan  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Pomingi","sequence":"additional","affiliation":[{"name":"Private Practice  Piacenza Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Letizia","family":"Tarantini","sequence":"additional","affiliation":[{"name":"EPIGET\u2010Epidemiology, Epigenetics and Toxicology Lab Department of Clinical Sciences and Community Health University of Milan  Milan 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