{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T03:48:00Z","timestamp":1781840880329,"version":"3.54.5"},"reference-count":60,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,10,24]],"date-time":"2017-10-24T00:00:00Z","timestamp":1508803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Hong Kong Research Grants Council","award":["HKU 768411M"],"award-info":[{"award-number":["HKU 768411M"]}]},{"name":"Hong Kong Research Grants Council","award":["17155216"],"award-info":[{"award-number":["17155216"]}]},{"name":"Modern Dental Laboratory\/HKU Endowment Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Periodontitis is closely linked with type 2 diabetes mellitus (T2DM) and endothelial dysfunction. This study investigated the effects of periodontal treatment on immuno\u2010inflammatory gene expression of endothelial progenitor cells (EPCs) in diabetic patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eighteen T2DM patients with moderate to severe chronic periodontitis were randomly assigned to the Treatment group with oral hygiene instruction (OHI), scaling and root debridement (<jats:italic>n<\/jats:italic>\u00a0=\u00a011), and Control group (<jats:italic>n<\/jats:italic>\u00a0=\u00a07) with OHI alone. Peripheral blood samples were taken for biochemical analysis and culture of EPCs at baseline and 6\u00a0months after the treatment. PCR array\u2010based profiling of 84 Toll<jats:italic>\u2010<\/jats:italic>like receptor signalling<jats:italic>\u2010<\/jats:italic>related genes in EPCs was firstly assessed for four randomly selected patients from the Treatment group. The differentially expressed genes were then further validated by qPCR in both groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All subjects in the Treatment group significantly improved their periodontal conditions. Among the 84 genes tested, IL\u20106 and IL\u20108 transcripts were significantly downregulated with over twofold change after the treatment, and this observation was further validated by qPCR in all subjects from both groups (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This preliminary study suggests that periodontal treatment may contribute to a notable reduction in immuno\u2010inflammatory gene expression measured by IL\u20106 and IL\u20108 transcripts in EPCs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12806","type":"journal-article","created":{"date-parts":[[2017,8,24]],"date-time":"2017-08-24T04:52:07Z","timestamp":1503550327000},"page":"1253-1263","source":"Crossref","is-referenced-by-count":12,"title":["Periodontal treatment modulates gene expression of endothelial progenitor cells in diabetic patients"],"prefix":"10.1111","volume":"44","author":[{"given":"Yi","family":"Wang","sequence":"first","affiliation":[{"name":"Discipline of Periodontology Faculty of Dentistry The University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hin Nam","family":"Liu","sequence":"additional","affiliation":[{"name":"Discipline of Periodontology Faculty of Dentistry The University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Zhen","sequence":"additional","affiliation":[{"name":"Division of Cardiology Department of Medicine Li Ka Shing Faculty of Medicine The University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai Hang","family":"Yiu","sequence":"additional","affiliation":[{"name":"Division of Cardiology Department of Medicine Li Ka Shing Faculty of Medicine The University of Hong Kong  Hong Kong SAR 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