{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:56:01Z","timestamp":1791888961956,"version":"4.3.4"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T00:00:00Z","timestamp":1515715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["S\u00e3o Paulo, S\u00e3o Paulo, Brazil, # 2013\/23743\u20109; 2013\/10354\u20104"],"award-info":[{"award-number":["S\u00e3o Paulo, S\u00e3o Paulo, Brazil, # 2013\/23743\u20109; 2013\/10354\u20104"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["Bras\u00edlia, Distrito Federal, Brasil, 441367\/2014\u20103"],"award-info":[{"award-number":["Bras\u00edlia, Distrito Federal, Brasil, 441367\/2014\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>This study evaluated the levels of sclerostin (SOST<\/jats:styled-content>) and Dickkopf (DKK<\/jats:styled-content>)\u20101 in the chronic periodontitis (CP<\/jats:styled-content>) associated with type 2 diabetes (DM<\/jats:styled-content>) and\/or smoking. Relationships between SOST<\/jats:styled-content>, DDK<\/jats:styled-content>1, RANKL<\/jats:styled-content>, OPG<\/jats:styled-content>, IL<\/jats:styled-content>\u20101\u00df, IL<\/jats:styled-content>\u20106 and TNF<\/jats:styled-content>\u2010\u03b1, and pathogens were assessed.<\/jats:p><\/jats:sec>Material and Methods<\/jats:title>The study population included non\u2010diabetic non\u2010smokers (control), non\u2010smokers with DM<\/jats:styled-content> (DM<\/jats:styled-content> group), non\u2010diabetic smokers (S group) and smokers with DM<\/jats:styled-content> (SDM<\/jats:styled-content> group), all with CP<\/jats:styled-content>. Serum and gingival levels of SOST<\/jats:styled-content>, DKK<\/jats:styled-content>1, RANKL<\/jats:styled-content>, OPG<\/jats:styled-content>, IL<\/jats:styled-content>\u20101\u00df, IL<\/jats:styled-content>\u20106 and TNF<\/jats:styled-content>\u2010\u03b1 were evaluated by multiplex immunoassay. Gene expressions of these biomarkers and subgingival levels of pathogens were assessed by qPCR<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>Gingival protein and\/or mRNA<\/jats:styled-content> levels of DKK<\/jats:styled-content>1 and SOST<\/jats:styled-content> were higher in subjects with DM<\/jats:styled-content> and\/or smoking than in controls (p<\/jats:italic>\u00a0<\u00a0.05). Serum levels of SOST<\/jats:styled-content> were higher in the DM<\/jats:styled-content> group than in controls (p<\/jats:italic>\u00a0<\u00a0.05). DKK<\/jats:styled-content>1 positively correlated with SOST<\/jats:styled-content> in the DM<\/jats:styled-content>, SDM<\/jats:styled-content> and control groups (p<\/jats:italic>\u00a0<\u00a0.05) at mRNA<\/jats:styled-content> levels. DKK<\/jats:styled-content>\u20101 and SOST<\/jats:styled-content> correlated with pathogens, especially in both groups with DM.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>SOST<\/jats:styled-content> and DKK<\/jats:styled-content>1 were upregulated in patients with CP<\/jats:styled-content> presenting DM<\/jats:styled-content> and\/or smoking. DM<\/jats:styled-content>, alone or with smoking, particularly influenced the correlations of SOST<\/jats:styled-content> and DKK<\/jats:styled-content>1 with each other and with the other biomarkers mostly at mRNA<\/jats:styled-content> levels, as well as with periodontal pathogens.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12854","type":"journal-article","created":{"date-parts":[[2017,12,16]],"date-time":"2017-12-16T10:46:55Z","timestamp":1513421215000},"page":"293-302","source":"Crossref","is-referenced-by-count":37,"title":["Antagonists of Wnt\/\u03b2\u2010catenin signalling in the periodontitis associated with type 2 diabetes and smoking"],"prefix":"10.1111","volume":"45","author":[{"given":"Tamires S.","family":"Miranda","sequence":"first","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4472-365X","authenticated-orcid":false,"given":"Marcelo H.","family":"Napimoga","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology and Molecular Biology S\u00e3o Leopoldo Mandic Institute and Research Center Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2293-3392","authenticated-orcid":false,"given":"Magda","family":"Feres","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia M.","family":"Marins","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele F.","family":"da Cruz","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9lio Doyle P.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8872-5943","authenticated-orcid":false,"given":"Poliana M.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.12.1736"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043638"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24487"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2011.01810.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22196"},{"key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.immuni.2016.01.008","article-title":"The Wnt antagonist Dickkopf\u20101 promotes pathological type 2 cell\u2010mediated inflammation","volume":"16","author":"Chae W. 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