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This study investigated differences in the expression of <jats:styled-content style=\"fixed-case\">AMP<\/jats:styled-content> coding genes obtained using a simple harvesting technique, gingival smear, between two groups of patients: chronic periodontitis subjects versus healthy ones.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Twenty\u2010three patients were enrolled in two groups: 12 were diagnosed with moderate or severe generalized chronic periodontitis, and 11 were diagnosed as clinically healthy. Gingival smears were retrieved and studied using reverse transcription\u2010quantitative <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>) after <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> purification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifteen gene expressions were obtained using real\u2010time <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. Three <jats:styled-content style=\"fixed-case\">AMP<\/jats:styled-content> genes, histatin 3 (<jats:styled-content style=\"fixed-case\">HTN<\/jats:styled-content>3), \u03b1\u2010defensin 4 (<jats:styled-content style=\"fixed-case\">DEFA<\/jats:styled-content>4) and lysozyme C (<jats:styled-content style=\"fixed-case\">LYZ<\/jats:styled-content>), presented different expression levels in periodontitis patients compared with healthy subjects. The relative expression level of <jats:styled-content style=\"fixed-case\">DEFA<\/jats:styled-content>4 appeared to be a protective factor against periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Gingival smears studied by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> may be used to assess the expression of <jats:styled-content style=\"fixed-case\">AMP<\/jats:styled-content>s coding genes. A lack of expression of <jats:styled-content style=\"fixed-case\">DEFA<\/jats:styled-content>4 could be a potential indicator of periodontitis status.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.12879","type":"journal-article","created":{"date-parts":[[2018,2,15]],"date-time":"2018-02-15T05:42:42Z","timestamp":1518673362000},"page":"524-537","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Antimicrobial peptide gene expression in periodontitis patients: A pilot study"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2470-9092","authenticated-orcid":false,"given":"Marie\u2010Laure","family":"Jourdain","sequence":"first","affiliation":[{"name":"EA 4691 Biomat\u00e9riaux et inflammation en site osseux (BIOS) SFR CAP\u2010Sant\u00e9 (FED 4231) Universit\u00e9 de Reims\u2010Champagne\u2010Ardenne  Reims France"},{"name":"UFR Odontologie  Reims France"},{"name":"P\u00f4le Odontologie 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