{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:10:04Z","timestamp":1787015404965,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T00:00:00Z","timestamp":1561420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE024160"],"award-info":[{"award-number":["R01DE024160"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005288","name":"Funda\u00e7\u00e3o Amaz\u00f4nia Paraense de Amparo \u00e0 Pesquisa","doi-asserted-by":"publisher","award":["2016\/21471\u20100"],"award-info":[{"award-number":["2016\/21471\u20100"]}],"id":[{"id":"10.13039\/501100005288","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>The purpose of this study was to determine inflammatory and epigenetic features following induction of oral and gut dysbiosis in experimental periodontitis in order to examine the interplay between oral and systemic infection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Periodontitis was induced in 6\u2010 to 8\u2010week\u2010old C57BL\/6 mice by (a) Ligature placement (Lig group) (oral challenge); (b)\n                      <jats:italic>P.\u00a0gingivalis<\/jats:italic>\n                      gavage (\n                      <jats:italic>Pg<\/jats:italic>\n                      group) (systemic challenge); and (c) the combination of the two models oral and systemic challenge (\n                      <jats:italic>Pg<\/jats:italic>\n                      \u00a0+\u00a0Lig). The duration of the experiment was 60\u00a0days, and the animals were then sacrificed for analyses. Alveolar bone loss was assessed, and a multiplex immunoassay was performed. Maxillae and gut tissues were immunostained for DNMT3b (de novo methylation marker), B and T lymphocyte attenuator (BTLA) and IL\u201018R1 (inflammation markers).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Pg<\/jats:italic>\n                      and\n                      <jats:italic>Pg<\/jats:italic>\n                      \u00a0+\u00a0Lig groups exhibited higher bone loss when compared to Sham. BAFF, VEGF, RANKL, RANTES and IP\u201010 were significantly higher with\n                      <jats:italic>Pg<\/jats:italic>\n                      gavage. Likewise, DNMT3b was overexpressed in both gut and maxilla after the\n                      <jats:italic>Pg<\/jats:italic>\n                      administration. The same pattern was observed for BTLA and IL\u201018R1 in gut tissues.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The systemic microbial challenge either alone or in combination with local challenge leads to distinct patterns of inflammatory and epigenetic features when compared to simply locally induced experimental periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.13151","type":"journal-article","created":{"date-parts":[[2019,5,27]],"date-time":"2019-05-27T05:55:05Z","timestamp":1558936505000},"page":"819-829","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Epigenetic and inflammatory events in experimental periodontitis following systemic microbial challenge"],"prefix":"10.1111","volume":"46","author":[{"given":"Daniela B.","family":"Palioto","sequence":"first","affiliation":[{"name":"Department of Periodontics, School of Dental Medicine University of Pennsylvania Philadelphia Pennsylvania"},{"name":"Department of OMS and Periodontology University of S\u00e3o Paulo \u2010 School of Dentistry of Ribeir\u00e3o Preto Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia S.","family":"Finoti","sequence":"additional","affiliation":[{"name":"Department of Periodontics, School of Dental Medicine University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis F.","family":"Kinane","sequence":"additional","affiliation":[{"name":"Division of Periodontology, School of Dental Medicine University of Geneva Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9218-7196","authenticated-orcid":false,"given":"Manjunatha","family":"Benakanakere","sequence":"additional","affiliation":[{"name":"Department of Periodontics, School of Dental Medicine University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1500496"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2013.05.002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910608500502"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.12.6574"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12231"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509335868"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058154"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034514557545"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40496-019-0208-4"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-13-107"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-015-1531-3"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1590\/1807-3107bor-2018.vol32.0035"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-015-1607-0"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1107830108"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3142"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-77572014ed004"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2007.01172.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3785"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2873"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2016.03.006"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2012.00663.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.10.006"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12105"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.13-LE-0548"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.04.005"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/76060"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12052"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.38"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-006-8967-1"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/idj.12264"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2014.140559"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0b013e31825719f3"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2018.1487741"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9272"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.4.1052-1059.1994"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12104"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijcp.13294"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-009-0142-7"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2886"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3402\/jom.v7.29143"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00199.x"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvx080"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1966.tb01860.x"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12808"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aac5380"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc13131"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12089"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2017.04.006"}],"container-title":["Journal of Clinical Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcpe.13151","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.13151","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcpe.13151","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.13151","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T07:22:50Z","timestamp":1694244170000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcpe.13151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,25]]},"references-count":48,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2019,8]]}},"alternative-id":["10.1111\/jcpe.13151"],"URL":"https:\/\/doi.org\/10.1111\/jcpe.13151","archive":["Portico"],"relation":{},"ISSN":["0303-6979","1600-051X"],"issn-type":[{"value":"0303-6979","type":"print"},{"value":"1600-051X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,25]]},"assertion":[{"value":"2019-01-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}