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Subgingival plaque samples collected from three healthy and three disease sites at weeks 0, 16, and 28 and from sites demonstrating disease progression were analysed for EBV, CMV, <jats:italic>P.\u00a0gingivalis (Pg), T.\u00a0forsythia (Tf),<\/jats:italic> and <jats:italic>T.\u00a0denticola (Td)<\/jats:italic> DNA using quantitative polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cytomegalovirus was detected in 0.3% (4\/1454) sites. EBV was present in 12.2% of healthy sites (89\/728) and 27.6% disease sites (201\/726; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.0001), but was in low copy number. Disease progression occurred in 28.4% of participants (23\/81) and developed predominantly at sites identified as diseased (75\/78; 96.2%). CMV and EBV were not associated with disease progression (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.13) regardless of treatment. In contrast, disease sites were associated with higher levels of <jats:italic>Pg, Td, Tf,<\/jats:italic> and total bacteria, and sites that exhibited disease progression were associated with an abundance of <jats:italic>Td<\/jats:italic> and <jats:italic>Tf<\/jats:italic> (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.04).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Disease progression was associated with Gram\u2010negative anaerobic bacteria; not EBV or CMV.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.13239","type":"journal-article","created":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T20:31:11Z","timestamp":1576873871000},"page":"442-450","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Relationship between herpesviruses and periodontal disease progression"],"prefix":"10.1111","volume":"47","author":[{"given":"Pinar","family":"Emecen\u2010Huja","sequence":"first","affiliation":[{"name":"Department of Oral Health Practice University of Kentucky Lexington Kentucky"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"Danaher","sequence":"additional","affiliation":[{"name":"Department of Oral Health Practice University of Kentucky Lexington Kentucky"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Dolphus R.","family":"Dawson","sequence":"additional","affiliation":[{"name":"Department of Oral Health Practice University of Kentucky Lexington Kentucky"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunmei","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Oral Health Research College of Dentistry University of Kentucky Lexington Kentucky"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard 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