{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T20:12:11Z","timestamp":1793391131103,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T00:00:00Z","timestamp":1581033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700992"],"award-info":[{"award-number":["81700992"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To explore the involvement of NOD\u2010like receptor protein 3 (NLRP3) inflammasome and M1 macrophage in root resorption (RR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A rat RR model was established by excessive orthodontic force. After different force\u2010loading time, the expression levels of NLRP3, caspase\u20101, and interleukin\u20101\u03b2 (IL\u20101\u03b2) and distribution of M1 macrophages were analysed by immunohistochemistry and immunofluorescence staining in vivo. Then, the mechanism of NLRP3 activation was further verified by macrophage and human periodontal ligament cell (hPDLC) co\u2010culture system in vitro. The production levels of NLRP3, caspase\u20101, pro\u2010caspase\u20101, and IL\u20101\u03b2 in M1 macrophages in the co\u2010culture system were detected by Western blot, and the polarization of CD68+IL\u20101\u03b2+ M1 macrophages was detected by immunofluorescence staining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In the rat RR model, NLRP3, caspase\u20101, IL\u20101\u03b2, and M1 macrophages were expressed in periodontal ligament, mainly concentrated around RR areas. Force\u2010pre\u2010treated hPDLCs promoted M1 macrophage polarization and the production of NLRP3, caspase\u20101, and IL\u20101\u03b2 in M1 macrophages in co\u2010culture system. When MCC950, an inhibitor of NLRP3 inflammasome, was added, NLRP3 activation and M1 macrophage polarization were inhibited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In periodontal tissues, hPDLCs stimulated by force promoted M1 macrophage polarization and increased IL\u20101\u03b2 production by activating NLRP3 inflammasome in M1 macrophages, thus initiating the occurrence of RR.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.13258","type":"journal-article","created":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T08:12:31Z","timestamp":1579853551000},"page":"451-460","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":271,"title":["NLRP3 inflammasome mediates M1 macrophage polarization and IL\u20101\u03b2 production in inflammatory root resorption"],"prefix":"10.1111","volume":"47","author":[{"given":"Jie","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University Qingdao China"},{"name":"School of Stomatology Qingdao University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University Qingdao China"},{"name":"School of Stomatology Qingdao University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Wan","sequence":"additional","affiliation":[{"name":"School of Stomatology Qingdao University Qingdao China"},{"name":"Department of Endodontics The Affiliated Hospital of Qingdao University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University Qingdao China"},{"name":"School of Stomatology Qingdao University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University Qingdao China"},{"name":"School of Stomatology Qingdao University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenda","family":"Meng","sequence":"additional","affiliation":[{"name":"School of Stomatology Qingdao University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yipeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Stomatology Qingdao University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9571-0958","authenticated-orcid":false,"given":"Chunmiao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University Qingdao China"},{"name":"School of Stomatology Qingdao University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2015.01.008"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40510-017-0177-x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1067\/mod.2003.42"},{"key":"e_1_2_9_5_1","first-page":"263","article-title":"Much more than M1 and M2 macrophages, there are also CD169(+) and TCR(+) macrophages","volume":"6","author":"Chavez\u2010Galan L.","year":"2015","journal-title":"Frontiers in Immunology"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3806"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.6.2095.bloodjournal8762095"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1303048"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00426"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v6.i5.526"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2018.09.004"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2019.01.003"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034514553817"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515589714"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2011.12.022"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-24350-x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2019.03.015"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12784"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034514555364"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijos.2017.34"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3452"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.4.601"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009252"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/cjx033"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002165"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/dom.12169"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2005.03.037"},{"key":"e_1_2_9_28_1","first-page":"175","article-title":"Orthodontically induced inflammatory root resorption. Part I: The basic science aspects","volume":"72","author":"Naphtali B.","year":"2002","journal-title":"Angle Orthodontist"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.19082\/7146"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000492886"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2339"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1184003"},{"key":"e_1_2_9_33_1","first-page":"271","article-title":"The effect of an angiogenic cytokine on orthodontically induced inflammatory root resorption","volume":"18","author":"Seifi M.","year":"2016","journal-title":"Cell Journal"},{"key":"e_1_2_9_34_1","first-page":"317","article-title":"Fibroblasts as sentinel cells. Synthesis of chemokines and regulation of inflammation","volume":"151","author":"Smith R. S.","year":"1997","journal-title":"American Journal of Pathology"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2018.03.002"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5460302"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep38308"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2577"}],"container-title":["Journal of Clinical Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcpe.13258","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.13258","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcpe.13258","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.13258","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T17:29:51Z","timestamp":1694021391000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcpe.13258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,7]]},"references-count":37,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1111\/jcpe.13258"],"URL":"https:\/\/doi.org\/10.1111\/jcpe.13258","archive":["Portico"],"relation":{},"ISSN":["0303-6979","1600-051X"],"issn-type":[{"value":"0303-6979","type":"print"},{"value":"1600-051X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,7]]},"assertion":[{"value":"2019-09-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}