{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:50:51Z","timestamp":1788310251787,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T00:00:00Z","timestamp":1602115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE019456"],"award-info":[{"award-number":["R01DE019456"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Previous data from our laboratory have demonstrated that localized aggressive periodontitis (LAP) patients produce elevated levels of pro\u2010inflammatory cytokines in response to TLR4 and TLR2 ligation compared to unrelated and periodontally healthy controls (HC). The aim of the present work is to evaluate the contribution of TLR\u2010related gene expression and miRNA regulation in LAP disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Peripheral blood mononuclear cells (PBMCs) from LAP and health control (HC) patients were isolated. Gene and miRNA expression involved in TLR signalling pathway and immunopathology were evaluated in unstimulated PBMCs by real\u2010time PCR (RT\u2010PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>TICAM\u20101<\/jats:italic> (<jats:italic>TRIF<\/jats:italic>), <jats:italic>FOS<\/jats:italic>, <jats:italic>IRA<jats:sc>K1<\/jats:sc><\/jats:italic>, <jats:italic><jats:sc>TLR2<\/jats:sc><\/jats:italic> and <jats:italic>CCL2<\/jats:italic> genes and the miRNAs miR\u20109\u20105p, miR\u2010155\u20105p and 203a\u20103p, miR\u2010147a, miR\u2010182\u20105p and miR\u2010183\u20105p were significantly up\u2010regulated in LAP compared to HC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Most of the genes and miRNAs overexpressed here are directly or indirectly related to immune response and inflammation. This profile supports our previous findings that suggests LAP patients have a \u201chyper\u2010responsive\u201d phenotype upon activation of TLR pathway by periodontal pathogens.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.13361","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T12:03:14Z","timestamp":1599048194000},"page":"1317-1325","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Dysregulation of genes and microRNAs in localized aggressive periodontitis"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9311-9630","authenticated-orcid":false,"given":"Jussara","family":"Gon\u00e7alves Fernandes","sequence":"first","affiliation":[{"name":"Department of Oral Health Practice College of Dentistry Center for Oral Health Research University of Kentucky  Lexington KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorri Ann","family":"Morford","sequence":"additional","affiliation":[{"name":"Division of Orthodontics Department of Oral Health Science College of Dentistry University of Kentucky  Lexington KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7526-4834","authenticated-orcid":false,"given":"Peter Lloyd","family":"Harrison","sequence":"additional","affiliation":[{"name":"Department of Periodontology College of Dentistry University of Florida  Gainesville FL USA"},{"name":"Division of Periodontology School of Dental Science Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodora","family":"Kompotiati","sequence":"additional","affiliation":[{"name":"Department of Periodontology College of Dentistry University of Florida  Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Periodontology College of Dentistry University of Florida  Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikramuddin","family":"Aukhil","sequence":"additional","affiliation":[{"name":"Department of Periodontology College of Dentistry University of Florida  Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon Margaret","family":"Wallet","sequence":"additional","affiliation":[{"name":"Department of Periodontology College of Dentistry University of Florida  Gainesville FL USA"},{"name":"Department of Oral and Craniofacial Health Sciences School of Dentistry The University of North Caroline  Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0948-1667","authenticated-orcid":false,"given":"Luciana","family":"Macchion Shaddox","sequence":"additional","affiliation":[{"name":"Department of Oral Health Practice College of Dentistry Center for Oral Health Research University of Kentucky  Lexington KY USA"},{"name":"Department of Periodontology College of Dentistry University of Florida  Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2013.120666"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0810909106"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3377"},{"issue":"4","key":"e_1_2_8_6_1","first-page":"294","article-title":"Potential risk for localized aggressive periodontitis in African American preadolescent children","volume":"39","author":"Childers N. 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