{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T17:52:48Z","timestamp":1778781168938,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T00:00:00Z","timestamp":1649116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K10011"],"award-info":[{"award-number":["20K10011"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K10169"],"award-info":[{"award-number":["19K10169"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To evaluate periodontal wound healing\/regeneration of one\u2010wall intra\u2010bony defects treated with recombinant human fibroblast growth factor\u20102 (rhFGF\u20102) and beta\u2010tricalcium phosphate (\u03b2\u2010TCP), carbonate apatite (CO<jats:sub>3<\/jats:sub>Ap), or deproteinized bovine bone mineral (DBBM) in dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The stability of rhFGF\u20102 adsorbed onto the bone substitutes was evaluated by Enzyme\u2010Linked Immunosorbent Assay (ELISA). One\u2010wall intra\u2010bony defects (5\u2009\u00d7\u20095\u2009\u00d7\u20095\u2009mm) created in five adult male beagle dogs were treated with rhFGF\u20102 alone (rhFGF\u20102), rhFGF\u20102 with \u03b2\u2010TCP (rhFGF\u20102\/\u03b2\u2010TCP), rhFGF\u20102 with CO<jats:sub>3<\/jats:sub>Ap (rhFGF\u20102\/CO<jats:sub>3<\/jats:sub>Ap), or rhFGF\u20102 with DBBM (rhFGF\u20102\/DBBM). Histological outcomes (e.g., linear length of new cementum adjacent to the newly formed bone with inserting collagen fibres [NA] as the primary outcome) were evaluated at 10\u2009weeks post surgery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significantly higher amount of rhFGF\u20102 was adsorbed onto CO<jats:sub>3<\/jats:sub>Ap compared with \u03b2\u2010TCP. Among the treatment groups, the rhFGF\u20102\/DBBM group showed the highest amount of periodontal tissue regeneration. The rhFGF\u20102\/DBBM group showed significantly greater formation of NA (3.22\u2009\u00b1\u20090.40\u2009mm) compared with rhFGF\u20102 (1.17\u2009\u00b1\u20091.00\u2009mm, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01) group. Additionally, new bone area in the rhFGF\u20102\/DBBM group (9.78\u2009\u00b1\u20092.30\u2009mm<jats:sup>2<\/jats:sup>) was significantly higher than that in the rhFGF\u20102 (5.08\u2009\u00b1\u20091.26\u2009mm<jats:sup>2<\/jats:sup>, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01), rhFGF\u20102\/\u03b2\u2010TCP (5.91\u2009\u00b1\u20091.27\u2009mm<jats:sup>2<\/jats:sup>, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), and rhFGF\u20102\/CO<jats:sub>3<\/jats:sub>Ap (6.51\u2009\u00b1\u20091.49\u2009mm<jats:sup>2<\/jats:sup>, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) groups. Slight ankylosis was found in the rhFGF\u20102\/\u03b2\u2010TCP (1\/9 sites), rhFGF\u20102\/CO<jats:sub>3<\/jats:sub>Ap (3\/10 sites), and rhFGF\u20102\/DBBM (1\/9 sites) groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Within their limitations, the present data indicate that DBBM seems to be a suitable carrier for rhFGF\u20102 and that rhFGF\u20102\/DBBM treatment promotes favourable periodontal regeneration compared with rhFGF\u20102, rhFGF\u20102\/\u03b2\u2010TCP, and rhFGF\u20102\/CO<jats:sub>3<\/jats:sub>Ap treatments in one\u2010wall intra\u2010bony defects.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.13619","type":"journal-article","created":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T23:19:32Z","timestamp":1648163972000},"page":"599-608","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Comparison of periodontal wound healing\/regeneration by recombinant human fibroblast growth factor\u20102 combined with \u03b2\u2010tricalcium phosphate, carbonate apatite, or deproteinized bovine bone mineral in a canine one\u2010wall intra\u2010bony defect model"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0663-0524","authenticated-orcid":false,"given":"Yoshinori","family":"Shirakata","sequence":"first","affiliation":[{"name":"Department of Periodontology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumiaki","family":"Setoguchi","sequence":"additional","affiliation":[{"name":"Department of Periodontology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kotaro","family":"Sena","sequence":"additional","affiliation":[{"name":"Department of Periodontology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2675-484X","authenticated-orcid":false,"given":"Toshiaki","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Periodontology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takatomo","family":"Imafuji","sequence":"additional","affiliation":[{"name":"Department of Periodontology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiya","family":"Shinohara","sequence":"additional","affiliation":[{"name":"Department of Periodontology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Iwata","sequence":"additional","affiliation":[{"name":"Department of Periodontology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyuki","family":"Noguchi","sequence":"additional","affiliation":[{"name":"Department of Periodontology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,4,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.11.032"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13385"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1993.tb00209.x"},{"issue":"4","key":"e_1_2_12_5_1","first-page":"321","article-title":"Clinical, radiographic, and histologic evaluation of human periodontal defects treated with Bio\u2010Oss and Bio\u2010Gide","volume":"18","author":"Camelo M.","year":"1998","journal-title":"International Journal of Periodontics and Restorative Dentistry"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034516632497"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12048"},{"key":"e_1_2_12_8_1","first-page":"53","article-title":"Bone loss in animals","volume":"9","author":"Draper H. 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