{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T03:06:56Z","timestamp":1771902416880,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T00:00:00Z","timestamp":1655337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016R1A2B2007246"],"award-info":[{"award-number":["2016R1A2B2007246"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study investigated whether a vitronectin\u2010derived peptide (VnP\u201016) prevents and\/or reverses alveolar bone resorption induced by ligature\u2010induced periodontitis in rodents and identified the underlying mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>We evaluated the effects of VnP\u201016 on osteogenic differentiation in human periodontal ligament cells (hPDLCs), lipopolysaccharide\u2010induced inflammatory responses in gingival fibroblasts, and immune response in T lymphocytes. Ligature\u2010induced periodontitis was induced by ligating the bilateral mandibular first molars for 14\u2009days in rats and for 7\u2009days in mice (<jats:italic>n<\/jats:italic>\u00a0=\u200910\/group). VnP\u201016 (100\u2009\u03bcg\/10\u00a0\u03bcl) was applied topically into the gingival sulcus of rats via intra\u2010sulcular injection, whereas the peptide (50\u2009\u03bcg\/5\u00a0\u03bcl) was administered directly into the gingiva of mice via intra\u2010gingival injection. To evaluate the preventive and therapeutic effects of VnP\u201016, micro\u2010computed tomography analysis and histological staining were then performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>VnP\u201016 promoted osteogenic differentiation of periodontal ligament cells and inhibited the production of lipopolysaccharide\u2010induced inflammatory mediators in gingival fibroblasts. Concomitantly, VnP\u201016 modulated the host immune response by reducing the number of receptor activator of NF\u2010\u03baB ligand (RANKL)\u2010expressing lipopolysaccharide\u2010stimulated CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells, and by suppressing RANKL and interleukin (IL)\u201017A production. Furthermore, local administration of VnP\u201016 in rats and mice significantly prevented and reversed alveolar bone loss induced by ligature\u2010induced periodontitis. VnP\u201016 enhanced osteoblastogenesis and simultaneously inhibited osteoclastogenesis and suppressed RANKL and IL\u201017A expression in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings suggest that VnP\u201016 acts as a potent therapeutic agent for preventing and treating periodontitis by regulating bone re\u2010modelling and immune and inflammatory responses.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.13671","type":"journal-article","created":{"date-parts":[[2022,5,30]],"date-time":"2022-05-30T07:35:08Z","timestamp":1653896108000},"page":"799-813","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A vitronectin\u2010derived peptide prevents and restores alveolar bone loss by modulating bone re\u2010modelling and expression of <scp>RANKL<\/scp> and <scp>IL\u201017A<\/scp>"],"prefix":"10.1111","volume":"49","author":[{"given":"Junbeom","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Periodontology, School of Dentistry Seoul National University  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong Ki","family":"Min","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Internal Medicine Konkuk University Medical Center  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cho Yeon","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Oral Biochemistry and Program in Cancer and Developmental Biology Dental Research Institute, School of Dentistry, Seoul National University  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun Ki","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Oral Biochemistry and Program in Cancer and Developmental Biology Dental Research Institute, School of Dentistry, Seoul National University  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung Youn","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Oral Biochemistry and Program in Cancer and Developmental Biology Dental Research Institute, School of Dentistry, Seoul National University  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9770-0705","authenticated-orcid":false,"given":"Byung\u2010Moo","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Oral Biochemistry and Program in Cancer and Developmental Biology Dental Research Institute, School of Dentistry, Seoul National University  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,16]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2013.05.002"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.6.2804-2809.1999"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2010.00347.x"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1993.64.5s.474"},{"key":"e_1_2_12_6_1","volume-title":"Disease control priorities in developing countries","author":"Bratthall D.","year":"2006"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2008.00463.x"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/731039"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2009.0864"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2337"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.11-S.2175"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2015.140520"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.4103\/0975-7406.100244"},{"key":"e_1_2_12_14_1","unstructured":"European Federation of Periodontology. 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