{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:15:10Z","timestamp":1789769710258,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T00:00:00Z","timestamp":1656374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81991503"],"award-info":[{"award-number":["81991503"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81991500"],"award-info":[{"award-number":["81991500"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81921002"],"award-info":[{"award-number":["81921002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Periodontitis (PD) is the sixth most prevalent disease around the world and is involved in the development and progression of multiple systemic diseases. Previous studies have reported that PD may aggravate liver injuries. The objective of this study was to investigate whether and how PD affects liver fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Ligature\u2010induced PD (LIP) was induced in male C57\/B6J mice, and sub\u2010gingival plaques (PL) from patients with PD were applied to mouse teeth. Liver fibrosis was induced by carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) injection. The mice were randomly divided into six groups: Oil, Oil+LIP, Oil+LIP+PL, CCl<jats:sub>4<\/jats:sub>, CCl<jats:sub>4<\/jats:sub>+LIP, and CCl<jats:sub>4<\/jats:sub>+LIP+PL. Alveolar bone resorption was evaluated by methylene blue staining. Hepatic function was analysed by serum alanine aminotransferase and hepatic hydroxyproline. Picrosirius red and \u03b1\u2010smooth muscle actin (SMA) staining were used to evaluate the fibrotic area. RNA sequencing and quantitative RT\u2010PCR were used to measure gene expression. Western blotting was used to measure protein levels. Flow cytometry was used to analyse the accumulation of immune cells. Mouse microbiota were analysed using 16S rRNA gene sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mice in the CCl<jats:sub>4<\/jats:sub>+LIP+PL group displayed higher serum alanine aminotransferase and hepatic hydroxyproline as well as more Picrosirius red\u2010positive and \u03b1\u2010SMA\u2010positive areas in liver samples than those of the CCl<jats:sub>4<\/jats:sub> group, suggesting that PD (LIP+PL) aggravated CCl<jats:sub>4<\/jats:sub>\u2010induced hepatic dysfunction and liver fibrosis. Consistently, the expression of fibro\u2010genic genes and the protein levels of transforming growth factor \u03b2 were much higher in the CCl<jats:sub>4<\/jats:sub>+LIP+PL group than in the CCl<jats:sub>4<\/jats:sub> group. Flow cytometry revealed that PD increased the accumulation of immune cells, including Kupffer cells, B cells, and Th17 cells, in the liver of mice with CCl<jats:sub>4<\/jats:sub> treatment. PD also increased the expression of inflammatory genes and activated pro\u2010inflammatory nuclear factor\u2010kappa B pathway in the livers of CCl<jats:sub>4<\/jats:sub>\u2010injected mice. Moreover, PD altered both oral and liver microbiota in CCl<jats:sub>4<\/jats:sub>\u2010injected mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PD aggravates CCl<jats:sub>4<\/jats:sub>\u2010induced hepatic dysfunction and fibrosis in mice, likely through the increase of inflammation and alteration of microbiota in the liver.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.13682","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T10:34:50Z","timestamp":1655462090000},"page":"1067-1078","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The combination of experimental periodontitis and oral microbiota from periodontitis patients aggravates liver fibrosis in mice"],"prefix":"10.1111","volume":"49","author":[{"given":"Lan","family":"Bai","sequence":"first","affiliation":[{"name":"Department of Periodontology, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Laboratory of Oral Microbiota and Systemic Diseases, Shanghai Ninth People's Hospital, College of Stomatology Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"National Center for Stomatology; National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Microbiota and Systemic Diseases, Shanghai Ninth People's Hospital, College of Stomatology Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"National Center for Stomatology; National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology  Shanghai 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