{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:10:45Z","timestamp":1790316645508,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T00:00:00Z","timestamp":1666051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772873"],"award-info":[{"award-number":["81772873"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970920"],"award-info":[{"award-number":["81970920"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970979"],"award-info":[{"award-number":["81970979"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Macrophages are closely involved in periodontitis. However, the molecular mechanism by which macrophages influence periodontitis is not well understood. We investigated the effects of phosphatase and tensin homologue (PTEN) on macrophage polarization, the underlying mechanism and the regulatory roles in periodontium regeneration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>PTEN expression in periodontitis macrophages was detected ex vivo. The effects of PTEN on macrophage polarization and the underlying mechanisms were investigated in vitro. We also analysed the ability of PTEN inhibitors to repair periodontitis in vivo in a ligature\u2010induced mouse model of periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Macrophage PTEN expression in periodontitis patients was significantly higher than that of controls. PTEN inhibition in macrophages induced alternative macrophage polarization, whereas PTEN overexpression facilitated classical polarization. PTEN inhibition facilitated activation of Akt1 while inhibiting expression of Akt2. Furthermore, Akt2 overexpression could rescue the effects of PTEN inhibition on NF\u2010\u03baB. Treatment with a PTEN inhibitor significantly attenuated the local inflammatory status and prevented alveolar bone resorption in the mouse model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings suggest that PTEN inhibition could induce alternative macrophage polarization by differentially regulating Akt1 and Akt2. This also changed a pro\u2010inflammatory microenvironment to an anti\u2010inflammatory environment by subsequently regulating the expression of NF\u2010\u03baB, thereby attenuating inflammatory alveolar bone resorption induced by ligature.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.13730","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T02:49:12Z","timestamp":1665456552000},"page":"220-231","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Phosphatase and tensin homologue determine inflammatory status by differentially regulating the expression of Akt1 and Akt2 in macrophage alternative polarization of periodontitis"],"prefix":"10.1111","volume":"50","author":[{"given":"Xiaowei","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Orthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Laboratory for Digital and Material Technology of Stomatology &amp; Beijing Key Laboratory of Digital Stomatology &amp; Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health &amp; NMPA Key Laboratory for Dental Materials  Beijing People's Republic of China"},{"name":"Department of Orthodontics Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yidi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Laboratory for Digital and Material Technology of Stomatology &amp; Beijing Key Laboratory of Digital Stomatology &amp; Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health &amp; NMPA Key Laboratory for Dental Materials  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haotian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Laboratory for Digital and Material Technology of Stomatology &amp; Beijing Key Laboratory of Digital Stomatology &amp; Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health &amp; NMPA Key Laboratory for Dental Materials  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Central Laboratory Peking University School and Hospital of Stomatology, No. 22 Zhongguancun South Avenue, Haidian District  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Laboratory for Digital and Material Technology of Stomatology &amp; Beijing Key Laboratory of Digital Stomatology &amp; Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health &amp; NMPA Key Laboratory for Dental Materials  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,18]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme-2019-0065"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/156800908784293659"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2017.01.005"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/fty076"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/6712591"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_202009_22806"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13156"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-007-0146-8"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4870"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/438085"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12273"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12961"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-01815-3"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2013.130078"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12464"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302167"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601515"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-03433-0"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2019.113674"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2081"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034520910127"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8BM00127H"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/2050-7771-2-1"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2017.03.006"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2019.10083"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12983"}],"container-title":["Journal of Clinical Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.13730","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcpe.13730","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.13730","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T06:00:48Z","timestamp":1692424848000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcpe.13730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,18]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1111\/jcpe.13730"],"URL":"https:\/\/doi.org\/10.1111\/jcpe.13730","archive":["Portico"],"relation":{},"ISSN":["0303-6979","1600-051X"],"issn-type":[{"value":"0303-6979","type":"print"},{"value":"1600-051X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,18]]},"assertion":[{"value":"2022-04-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}