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This makes it possible to use EA as an integrated treatment for periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>In this study, EA was applied to the ST36 acupoints in a ligature\u2010induced periodontitis (LIP) mouse model. Then the effects of EA on periodontal myeloid cells, cytokines, and the microbiome were comprehensively analysed using flow cytometry, quantitative Polymerase Chain Reaction (PCR), and 16\u2009S sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Results demonstrated that EA could significantly relieve periodontal bone resorption. EA also suppressed the infiltration of macrophages and neutrophils, reduced gene expression of the pro\u2010inflammatory cytokines IL\u20101\u03b2, IL\u20106, IL\u201017 and TNF\u2010\u03b1, and increased expression of the anti\u2010inflammatory factors IL\u20104 and IL\u201010 in periodontal tissues. Moreover, composition of the periodontal microbiome was regulated by EA, finding that complex of microbiota, including supragingival\n                      <jats:italic>Veillonella<\/jats:italic>\n                      , subgingival\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      , and subgingival\n                      <jats:italic>Erysipelatoclostridium,<\/jats:italic>\n                      were significantly reduced. Meanwhile, nitrate and nitrate\u2010related activities of subgingival microbiota were reversed. Network analysis revealed close relationships among\n                      <jats:italic>Veillonella<\/jats:italic>\n                      ,\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      , and\n                      <jats:italic>Bacteroides<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study indicates that EA can effectively alleviate inflammation and bone resorption in LIP mice, potentially via the regulation of myeloid cells, cytokines, and periodontal microbiome.<\/jats:p>\n                  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