{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T11:03:33Z","timestamp":1788260613491,"version":"build-2803163510"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T00:00:00Z","timestamp":1678233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["654\/20"],"award-info":[{"award-number":["654\/20"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["632\/20"],"award-info":[{"award-number":["632\/20"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To reveal the heterogeneity of ex vivo\u2010cultured human mesenchymal stromal cells derived from either masticatory or lining oral mucosa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Cells were retrieved from the lamina propria of the hard palate and alveolar mucosa of three individuals. The analysis of transcriptomic\u2010level differences was accomplished using single\u2010cell RNA sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cluster analysis clearly distinguished between cells from the masticatory and lining oral mucosa, and revealed 11 distinct cell sub\u2010populations, annotated as fibroblasts, smooth muscle cells or mesenchymal stem cells. Interestingly, cells presenting a mesenchymal stem cell\u2010like gene expression pattern were predominantly found in masticatory mucosa. Although cells of masticatory mucosa origin were highly enriched for biological processes associated with wound healing, those from the lining oral mucosa were highly enriched for biological processes associated with the regulation of epithelial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our previous work had shown that cells from the lining and masticatory oral mucosae are phenotypically heterogeneous. Here, we extend these findings to show that these changes are not the result of differences in averages but rather represent two distinct cell populations, with mesenchymal stem cells more common in masticatory mucosa. These features may contribute to specific physiological functions and have relevance for potential therapeutic interventions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.13799","type":"journal-article","created":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T06:33:37Z","timestamp":1677825217000},"page":"807-818","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Single\u2010cell transcriptomic analysis of oral masticatory and lining mucosa\u2010derived mesenchymal stromal cells"],"prefix":"10.1111","volume":"50","author":[{"given":"Ilan","family":"Pinkhasov","sequence":"first","affiliation":[{"name":"Department of Oral Biology, Goldschleger School of Dental Medicine, Sackler Faculty of Medicine Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liron","family":"Kabakov","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Goldschleger School of Dental Medicine, Sackler Faculty of Medicine Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos E.","family":"Nemcovsky","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Oral Implantology, Goldschleger School of Dental Medicine, Sackler Faculty of Medicine Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miron","family":"Weinreb","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Goldschleger School of Dental Medicine, Sackler Faculty of Medicine Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pnina","family":"Schlesinger","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Goldschleger School of Dental Medicine, Sackler Faculty of Medicine Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omer","family":"Bender","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Goldschleger School of Dental Medicine, Sackler Faculty of Medicine Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3668-8647","authenticated-orcid":false,"given":"Maayan","family":"Gal","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Goldschleger School of Dental Medicine, Sackler Faculty of Medicine Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Z.","family":"Bar","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Goldschleger School of Dental Medicine, Sackler Faculty of Medicine Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8010-8921","authenticated-orcid":false,"given":"Evgeny","family":"Weinberg","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Goldschleger School of Dental Medicine, Sackler Faculty of Medicine Tel Aviv University  Tel Aviv Israel"},{"name":"Department of Periodontology and Oral Implantology, Goldschleger School of Dental Medicine, Sackler Faculty of Medicine Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,8]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12728"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/jomfp.JOMFP_146_15"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0709"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.62810"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jopr.12316"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-018-0850-0"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240600855905"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/7154327"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2020.151123"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12072"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aay6853"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240500319234"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01542.x"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14891"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13532"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1971.tb00619.x"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1975.tb00001.x"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.667221"},{"issue":"1","key":"e_1_2_12_20_1","first-page":"159","article-title":"Tissue interactions in embryonic mouse tooth germs. I. Reorganization of the dental epithelium during tooth\u2010germ reconstruction","volume":"24","author":"Kollar E. J.","year":"1970","journal-title":"Journal of Embryology and Experimental Morphology"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1619"},{"key":"e_1_2_12_22_1","first-page":"b18","article-title":"Exploring scarless healing of oral soft tissues","volume":"77","author":"Larjava H.","year":"2011","journal-title":"Journal of the Canadian Dental Association"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2021.11.005"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0751-7"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12960"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.425"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.01.013"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2010.090566"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2017.08.005"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.637725"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2009.04.002"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-7-29"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12070"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3192"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt413"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08683"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1281-2"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jncimonographs.a003443"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.05.031"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11081095"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12446"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2019.08.002"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11081165"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2010.0523"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.650"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-020-03304-6"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.05.013"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2009.00531.x"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100141"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-01855-9"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1159\/000360276"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1593"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gpb.2022.01.005"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034512461016"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14049"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc21042"}],"container-title":["Journal of Clinical Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.13799","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcpe.13799","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.13799","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T20:14:58Z","timestamp":1692562498000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcpe.13799"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,8]]},"references-count":56,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1111\/jcpe.13799"],"URL":"https:\/\/doi.org\/10.1111\/jcpe.13799","archive":["Portico"],"relation":{},"ISSN":["0303-6979","1600-051X"],"issn-type":[{"value":"0303-6979","type":"print"},{"value":"1600-051X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,8]]},"assertion":[{"value":"2022-11-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}