{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:06:43Z","timestamp":1792026403873,"version":"4.4.0"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T00:00:00Z","timestamp":1697673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DE028915"],"award-info":[{"award-number":["R01DE028915"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009238","name":"Virginia Commonwealth University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009238","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To determine the effects of RVX\u2010208, a selective bromodomain and extra\u2010terminal domain (BET) inhibitor targeting bromodomain 2 (BD2), on periodontal inflammation and bone loss.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Macrophage\u2010like cells (RAW264.7) and human gingival epithelial cells were challenged by <jats:italic>Porphyromonas gingivalis<\/jats:italic> (<jats:italic>Pg<\/jats:italic>) with or without RVX\u2010208. Inflammatory gene expression and cytokine production were measured by reverse transcription polymerase chain reaction and enzyme\u2010linked immunosorbent assay, respectively. RAW264.7 cells were induced to osteoclast differentiation. After RVX\u2010208 treatment, osteoclast differentiation was evaluated by histology, tartrate\u2010resistant\u2010acid\u2010phosphatase (TRAP) activity and the expression of osteoclast\u2010specific genes. The effect of RVX\u2010208 on osteoclast transcriptome was studied by RNA sequencing. Periodontitis was induced in rats by ligature and local RVX\u2010208 treatment was administered every other day. Alveolar bone loss was measured by micro\u2010computed tomography.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RVX\u2010208 inhibited inflammatory gene expression and cytokine production in <jats:italic>Pg<\/jats:italic>\u2010infected cells. Osteoclast differentiation was inhibited by RVX\u2010208, as evidenced by reduced osteoclast number, TRAP activity and osteoclast\u2010specific gene expression. RVX\u2010208 displayed a more selective and less profound suppressive impact on transcriptome compared with pan\u2010BET inhibitor, JQ1. RVX\u2010208 administration prevented the alveolar bone loss in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>RVX\u2010208 regulated both upstream (inflammatory cytokine production) and downstream (osteoclast differentiation) events that lead to periodontal tissue destruction, suggesting that it may be a promising \u2018epi\u2010drug\u2019 for the prevention of periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.13887","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T11:05:16Z","timestamp":1697713516000},"page":"1658-1669","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Selective <scp>BET<\/scp> inhibitor <scp>RVX<\/scp>\u2010208 ameliorates periodontal inflammation and bone loss"],"prefix":"10.1111","volume":"50","author":[{"given":"Mingxu","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Periodontics, School 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