{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T02:47:25Z","timestamp":1791859645734,"version":"4.3.4"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T00:00:00Z","timestamp":1703030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["#305077\/2021\u20100"],"award-info":[{"award-number":["#305077\/2021\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      We sought to investigate the release of neutrophil extracellular traps (NETs) in neutrophils from individuals with rheumatoid arthritis (RA) and controls and compare the presence of NETs in gingival tissues according to periodontal status. Also, the association between single nucleotide polymorphisms (SNPs) of the peptidyl arginine deaminase type 4 (\n                      <jats:italic>PADI4<\/jats:italic>\n                      ) gene and the GTG haplotype with RA, periodontitis and NETs was evaluated in vitro.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Peripheral neutrophils were isolated by density gradient, and NET concentration was determined by the PicoGreen method. Immunofluorescence was studied to identify NETs by co\u2010localization of myeloperoxidase (MPO)\u2010citrullinated histone H3 (H3Cit). Genotyping for SNPs (\n                      <jats:italic>PADI4<\/jats:italic>\n                      _89;\n                      <jats:italic>PADI4<\/jats:italic>\n                      _90;\n                      <jats:italic>PADI4<\/jats:italic>\n                      _92; and\n                      <jats:italic>PADI4<\/jats:italic>\n                      _104) was performed in 87 individuals with RA and 111 controls.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The release of NETs in vitro was significantly higher in individuals with RA and periodontitis and when stimulated with\n                      <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                      . Gingival tissues from subjects with RA and periodontitis revealed increased numbers of MPO\u2010H3Cit\u2010positive cells. Individuals with the GTG haplotype showed a higher release of NETs in vitro and worse periodontal parameters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The release of NETs by circulating neutrophils is associated with RA and periodontitis and is influenced by the presence of the GTG haplotype.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.13921","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T03:31:10Z","timestamp":1703043070000},"page":"452-463","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Neutrophil extracellular traps in rheumatoid arthritis and periodontitis: Contribution of\n                    <scp>\n                      <i>PADI4<\/i>\n                    <\/scp>\n                    gene polymorphisms"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8164-2122","authenticated-orcid":false,"given":"Sic\u00edlia Rezende","family":"Oliveira","sequence":"first","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry Federal University of Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6599-3950","authenticated-orcid":false,"given":"Jos\u00e9 Alcides Almeida","family":"de Arruda","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry Federal University of Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2474-2773","authenticated-orcid":false,"given":"Ayda Henriques","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2037-9532","authenticated-orcid":false,"given":"Larissa Marques","family":"Bemquerer","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry Federal University of Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rayssa Maria Soalheiro","family":"de Souza","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry Federal University of Minas Gerais  Belo Horizonte Minas Gerais 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